数字农科院2.0

Modification of starch composition, structure and properties through editing of TaSBEIIa in both winter and spring wheat varieties by CRISPR/Cas9

文献类型： 外文期刊

作者： Li Jingying;Jiao Guiai;Sun Yongwei;Chen Jun;Zhong Yingxin;Yan Lei;Jiang Dong;Ma Youzhi;Xia Lanqin

作者机构：

关键词： amylose; CRISPR/Cas9; resistant starch (RS); starch; starch -branching enzyme (SBE); wheat (Triticum aestivum L.)

期刊名称： Plant Biotechnology Journal

ISSN： 1467-7644

年卷期： 2021 年 19 卷 5 期

页码：

收录情况： JCR（2021版）

摘要： Foods high in amylose content and resistant starch (RS) offer great potential to improve human health and lower the risk of serious noninfectious diseases. Common wheat (Triticum aestivum L.) is a major staple food crop globally. However, the RS contents in the grains of modern wheat varieties are low. Here, we report the generation of high-amylose wheat through targeted mutagenesis of TaSBEIIa in a modern winter wheat cv Zhengmai 7698 (ZM) and a spring wheat cv Bobwhite by CRISPR/Cas9, respectively. We generated a series of transgene-free mutant lines either with partial or triple-null TasbeIIa alleles in ZM and Bobwhite, respectively. Analyses of starch composition, structure and properties revealed that the effects of partial or triple-null alleles were dosage dependent with triple-null lines demonstrated more profound impacts on starch composition, fine structures of amylopectin and physiochemical and nutritional properties. The flours of triple-null lines possessed significantly increased amylose, RS, protein and soluble pentosan contents which benefit human health. Baking quality analyses indicated that the high-amylose flours may be used as additives or for making cookies. Collectively, we successfully modified the starch composition, structure and properties through targeted mutagenesis of TaSBEIIa by CRISPR/Cas9 in both winter and spring wheat varieties and generated transgene-free high-amylose wheat. Our finding provides deep insights on the role of TaSBEIIa in determining starch composition, structure, properties and end-use quality in different genetic backgrounds and improving RS content with multiple breeding and end-use applications in cereal crop species through genome editing for health benefits. © 2020 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

分类号：

  • 相关文献

[1]Modification of starch composition, structure and properties through editing of TaSBEIIa in both winter and spring wheat varieties by CRISPR/Cas9. Li Jingying,Jiao Guiai,Sun Yongwei,Chen Jun,Zhong Yingxin,Yan Lei,Jiang Dong,Ma Youzhi,Xia Lanqin. 2021

[2]Generation Of High-Amylose Rice Through C.rispr/Cas9-Mediated Targeted Mutagenesis Of S tarch Branching Enzymes. Sun, Yongwei,Liu, Zupei,Xia, Lanqin,Zhao, Yunde,Zhang, Xin,Li, Jingying,Du, Wenming,Du, Jinlu,Francis, Frederic,Jiao, Guiai,Guo, Xiuping. 2017

[3]Generation Of High-Amylose Rice Through CRISPR/Cas9-Mediated Targeted Mutagenesis Of Starch Branching Enzymes. Sun, YW, Jiao, GA, Liu, ZP, Zhang, X, Li, JY, Guo, XP, Du, WM, Du, JL, Francis, F, Zhao, YD, Xia, LQ. 2017

[4]Comparison of quercetin and rutin inhibitory influence on Tartary buckwheat starch digestion in vitro and their differences in binding sites with the digestive enzyme. Wang L., Wang L., Wang T., Li Z., Gao Y., Cui S.W., Qiu J.. 2022

[5]ZBED6基因对巴马香猪脾发育的作用机制分析. 王圣楠,王丹丹,田文杰,浦亚斌,潘登科,邢向阳,马月辉,蒋琳. 2021

[6]基于CRISPR/Cas9基因编辑系统构建敲除DDX5基因的猪肾细胞系. 金彩燕,吴金恩,何童童,孙世琪,郭慧琛,张小丽. 2021

[7]采用 CRISPR/Cas9 创制黄籽高抗裂角油菜杂交种. 蔡雅婷,,郝梦宇,,李一林,,温运飞,,王会,,汪文祥,,付丽,,胡琼,,成洪涛,,梅德圣. 2024

[8]Genome-Wide Identification And Expression Analysis O.f Cutinase Gene Family I n Rhizoctonia Cerealis And Functional Study Of An Active Cutinase Rccut1 In The Fungal Wheat Interaction. Lu, L, Rong, W, Massart, S, Zhang, ZY. 2018

[9]Selenium distribution, translocation and speciation in wheat (Triticum aestivum L.) after foliar spraying selenite and selenate. Di X., Qin X., Zhao L., Liang X., Xu Y., Sun Y., Huang Q.. 2023

[10]Global Characterization Of GH10 Family Xylanase Genes In Rhizoctonia Cerealis And Functional Analysis of Xylanase RcXYN1 During Fungus Infection In Wheat. 张增艳,,卢霖. 2020

[11]Interactions Between Rice Amylose And A.roma Compounds And Their E ffect On Rice Fragrance Release. Chen, Long,Chen, Long,Cai, Canxin,Jin, Zhengyu,Cai, Canxin,Tian, Yaoqi,Ma, Rongrong,Ma, Rongrong,Jin, Zhengyu,Zhang, Haihua,Tian, Yaoqi. 2019

[12]PREDICTION OF THE TOTAL STARCH AND AMYLOSE CONTENT IN BARLEY USING NEAR-INFRARED REFLECTANCE SPECTROSCOPY. Ping, Hua,Wang, Jihua,Ren, Guixing. 2013

[13]Shear degradation of corn starches with different amylose contents. Liu, Xingxun,Li, Ming,Zhou, Sumei,Xiao, Xiaoming,Yu, Long,Liu, Peng,Xie, Fengwei.

[14]Physicochemical characterization of sweet potato starches popularly used in Chinese starch industry. Abegunde, Oluwaseyi Kemi,Mu, Tai-Hua,Chen, Jing-Wang,Deng, Fu-Ming.

[15]Insights into molecular structure and digestion rate of oat starch. Xu, Jinchuan,Wang, Shuo,Wang, Shujun,Xu, Jinchuan,Kuang, Qirong,Zhou, Sumei,Liu, Xingxun,Wang, Kai.

[16]Direct enantioseparation of nitrogen-heterocyclic pesticides on amylose-tris-(5-chloro-2-methylphenylcarbamate) by reversed-phase high-performance liquid chromatography. Yang, Wenwen,Qiu, Jing,Chen, Tianjin,Yang, Shuming,Yang, Wenwen,Qiu, Jing,Chen, Tianjin,Yang, Shuming,Yang, Wenwen,Hou, Shicong.

[17]Hierarchical Structure And Thermal Behavior O.f Hydrophobic Starch-Based Films W ith Different Amylose Contents. Fu, LL, Zhu, J, Zhang, SY, Li, XX, Zhang, BJ, Pu, HY, Li, L, Wang, Q. 2018

[18]Important Sensory Properties Differentiating Premium Rice Varieties. Elaine T. Champagne *, Karen L. Bett-Garber , Melissa A. Fitzgerald , Casey C. Grimm , Jeanne Lea , Ken’ichi Ohtsubo , Supanee Jongdee , Lihong Xie , Priscila Zaczuk Bassinello , Adoracion Resurreccion , Rauf Ahmad , Fatemah Habibi , Russell Reinke. 2010

[19]Alternative splicing of OsGS1;1 affects nitrogen-use efficiency, grain development, and amylose content in rice. Liu, Xiaolan,Tian, Yunlu,Chi, Wenchao,Zhang, Hanzhi,Yu, Jun,Chen, Gaoming,Wu, Wei,Jiang, Xingzhou,Wang, Saisai,Lin, Zhixi,Xuan, Wei,Ye, Jian,Wang, Baoxiang,Liu, Yan,Sun, Zhiguang,Xu, Dayong,Wang, Chunming,Wan, Jianmin. 2022

[20]Effect of climate warming on the grain quality of early rice in a double-cropped rice field: A 3-year measurement. Yong Wang,Taotao Yang,Ruoyu Xiong,Yanhua Zeng,Jun Zhang,Feiyu Tang,Yongjun Zeng,Shan Huang. 2023

作者其他论文 更多>>