数字农科院2.0

Rapid Milk Somatic Cell Counting Based on a Customized Multichamber Chip

文献类型： 外文期刊

作者： Sun, Xiaoyun;Zhao, Ruiming;Wang, Nan;Zhang, Juan;Xie, Ruibin;Xiao, Bin;Wen, Shuang;Gao, Jie;Chen, Ailiang

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关键词： milk somatic cell;count;customized multichamber chip;fluorescent dye

期刊名称： ACS AGRICULTURAL SCIENCE & TECHNOLOGY

ISSN：

年卷期： 2022 年

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收录情况： 不在遴选数据库范围

摘要： Rapid and accurate detection of milk somatic cell count (SCC) is of great significance for monitoring dairy cow udder health and ensuring milk quality. The conventional direct microscopic somatic cell counting method is low cost, but the accuracy of measured results is usually poor. While the flow cytometry method is widely used, which can accurately generate milk somatic cell count, the equipment cost is usually high. To address these issues, a customized multichamber chip was designed for milk somatic cell counting, combining cell fluorescent staining reagents and specially designed cell counters. The validation testing results with 30 samples with different somatic cell counts showed that the proposed method was highly consistent with two commercial milk somatic cell counters. In addition, the microchamber chip for SCC showed good reproducibility with the coefficients of variation <10%. Therefore, the easy-to-use multichamber chip-based SCC method provides a useful tool for tracking daily milk quality and controlling the incidence of mastitis.

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