数字农科院2.0

Synergistic Control of Megalurothrips usitatus through Sequential RNAi Targeting Novel Lethal Genes and Serratia marcescens HvSm-1

文献类型： 外文期刊

作者： Chen, Jian;Li, Jianxing;Zhong, Zexin;Yu, Yuanbin;Yang, Chunxiao;Zhang, Chaoqun;Tang, Riyuan;Xie, Wen;Zhang, Youjun;Pan, Huipeng

作者机构：

关键词： Megalurothrips usitatus;RNA interference;gene silencing;Serratia marcescens;synergisticeffects;dsRNA stability

期刊名称： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

ISSN： 0021-8561

年卷期： 2025 年

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： The phytophagous thrip, Megalurothrips usitatus is a destructive pest of cowpea. This study developed an integrated management strategy combining RNA interference (RNAi) and the entomopathogen Serratia marcescens HvSm-1. Seed feeding emerged as the optimal dsRNA delivery method. Screening identified four lethal target genes (Mu alpha COPI, MuNICE3, MuSrp54k, MuRop). Silencing significantly increased mortality (nymphs: 23.1-34.8%; adults: 25.5-33.3%). The bacterium HvSm-1 alone caused high, dose-dependent mortality. A sequential application of dsRNA followed by HvSm-1 induced synergistic or additive mortality, significantly enhancing control efficacy in both life stages. In nymphs, mortality reached 82.1-88.4%. Synergistic effects were observed for dsMuaCOPI, dsMuNICE3, and dsMuSrp54k with HvSm-1. In adults, mortality ranged from 51.7% to 58.0%. Synergy occurred for dsMuaCOPI, dsMuSrp54k, and dsMuRop with HvSm-1. However, direct mixing was ineffective as bacterial nucleases degraded dsRNA within hours. This work demonstrates the potential of sequential RNAi-microbial biocontrol while identifying dsRNA stability as a critical challenge for future formulation.

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