数字农科院2.0

Global Scale Insights into Genetic Variation in Mitochondrial cox1 and nad1 Genes of Clonorchis sinensis

文献类型： 外文期刊

作者： Shahbaz Ul Haq;Talha Javaid;Majed H. Wakid;Muhammad Wasim Usmani;Muhammad Saqib;Muhammad Abdullah Malik;Shujaat Hussain;Safia Obaidur Rab;Mohd Saeed;Asif Ali Butt;Fenfei Gao;Hong Bin Yan;Li Li;Mughees Aizaz Alvi

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关键词： Clonorchis sinensis;Diversity indices;Epidemiology;Haplotypes;Neutrality indices;Population structure

期刊名称： Pakistan Veterinary Journal

ISSN： 0253-8318

年卷期： 2025 年 45 卷 2 期

页码：

收录情况： SCIE（2025版）

摘要： Clonorchiasis is a food-borne zoonoses caused by Clonorchis (C.) sinensis. Human infection occurs through consumption of raw or undercooked fish containing metacercaria, an infective stage that can persist for 20 years, leading to chronic diseases. Few studies have been conducted on C. sinensis based on population structure, and even fewer have been conducted on molecular characterization. The goal of the current study was to provide epidemiological information on the global population structure of the parasite. After obtaining the cox1 and nad1 genes sequences from the NCBI GenBank database, median-joining networks were constructed using PopArt software. The neutrality and diversity indexes were computed using the DnaSp software. We also calculated neutrality and diversity indices based on host and geographic location. In the NCBI database, there were 209 nad1 and 275 cox1 gene sequences. After removing short gene sequences, bioinformatic analysis was performed using 251 cox1 and 189 nad1 gene sequences. The cox1 and nad1 genes were shown to have 40 and 42 parsimony informative sites, 87 and 73 haplotypes, and 82 mutations, respectively. We found negative and statistically significant Tajima's D (cox1=-2.41336 and nad1=-2.50463), Fu’s Fs values were negative but did not reach statistical significance (cox1=-32.908, nad1=-107.788), and high diversity (cox1 Hd=0.948, π=0.00317; nad1 Hd=0.9192, π=0.00293). South Korean isolates exhibited the highest haplotype diversity for both genes, with Chinese and Russian isolates following closely behind. A statistically significant overall negative Tajima's D value was found for both genes based on host-wise data analysis. According to the study's findings, high diversity and negative neutrality indices indicate that C. sinensis population diversity will increase globally. The results of the investigation will contribute to the current understanding of the population structure of C. sinensis, for which there is a dearth of data. To have a better understanding of the parasite's epidemiology, we recommend conducting more research using a number of genetic markers.

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