Molecular identification and development of an infectious cDNA clone of Trichosanthes kirilowii-infecting cucurbit mild mosaic virus
文献类型: 外文期刊
作者: Cheng Chen;Min Du;Yaqin Wang;Xueping Zhou;Xiuling Yang
作者机构:
关键词: Cucurbit mild mosaic virus;Cucurbitaceae;Fabavirus;Infectious clone;Mechanical transmission
期刊名称: Virology
ISSN: 0042-6822
年卷期: 2023 年 588 卷
页码:
收录情况: SCIE(2023版)
摘要: Trichosanthes kirilowii has been mainly grown for use in traditional Chinese medicine. In this study, cucurbit mild mosaic virus (CuMMV) belonging to the genus Fabavirus was identified from T. kirilowii plants. CuMMV possesses a segmented, bipartite linear single-stranded RNA genome composed of RNA1 and RNA2. Sequence analysis showed that each genomic segment shares the highest sequence similarity with those of CuMMV isolated from pumpkin. A full-length infectious cDNA clone of CuMMV was further constructed and was found to induce typical symptoms in T. kirilowii, Cucumis sativus, C. melo, Citrullus lanatus, and Cucurbita pepo. The sap inoculum derived from the infectious cDNA clone of CuMMV could be mechanically transmitted and reproduce similar symptoms in the tested plants. This is the first report on the construction of a biologically active, full-length infectious cDNA clone of CuMMV, which will provide a useful tool in understanding CuMMV-encoded proteins and plant-CuMMV interactions.
分类号:
- 相关文献
作者其他论文 更多>>
-
SARS-CoV-2 rORF3, a novel microprotein, impairs interferon-β production by targeting RanBP2 to inhibit p65 nuclear import
作者:Yucong Huang;Jing Jiang;Qingting Xiao;Yajie Wang;Gulinaziya Abulaike;Ayinuer Maimaiti;Pan Gong;Xiaohuang Lin;Fangfang Li;Xueping Zhou;Alexander F. Palazzo;Qingtang Shen
关键词:Innate immunity;Microprotein;p65;RanBP2;rORF3;Severe acute respiratory syndrome coronavirus 2
-
Viral RNA polymerase as a SUMOylation decoy inhibits RNA quality control to promote potyvirus infection
作者:Linhao Ge;Mingxuan Jia;Hongying Shan;Weifang Gao;Lu Jiang;Hongguang Cui;Xiaofei Cheng;Marilyne Uzest;Xueping Zhou;Aiming Wang;Fangfang Li
关键词:Viral RNA polymerase;; SUMOylation decoy;;RNA quality control ;;potyvirus infection
-
RNA modifications in plant biotic interactions
作者:Linhao Ge;Fuan Pan;Mingxuan Jia;Delphine M. Pott;Hao He;Hongying Shan;Rosa Lozano-Durán;Aiming Wang;Xueping Zhou;Fangfang Li
关键词:m6A;m6A core components;N6-methyladenosine;plant biotic stress;plant–insect interactions;plant–microbe interactions;RNA modifications
-
Establishment of efficient Trichosanthes mottle mosaic virus-derived gene silencing in cucurbit plants
作者:Cheng Chen;Zhu Fang;Min Du;Changkai Yang;Yukui Yang;Xueping Zhou;Xiuling Yang
关键词:Cucurbits;Flower;Trichosanthes mottle mosaic virus;Virus-induced gene silencing
-
Multiplex lateral flow immunoassay for simultaneous detection and specific discrimination of Xoo and Xoc in rice plants
作者:Cui Zhang;Xi Zhang;Nairu Liu;Jie Dong;Weijia Mao;Zhaoli Liu;Xueping Zhou;Jianxiang Wu
关键词:AuNPs;Multiplex lateral flow immunoassay;Simultaneous detection;Xoc;Xoo
-
Rice ragged stunt virus Pns10 induces mitochondrial-mediated apoptosis to promote viral infection in Nilaparvata lugens through disrupting the NlNDUFS1-NlPHB2 interaction
作者:Lianshun Zheng;Shuai Fu;Ming Zeng;Liyan Li;Dan Wang;Shibo Gao;Yunge Zhang;Cui Zhang;Shifang Fei;Xuan Ye;Lele Chen;Qianhui Chen;Yaqin Wang;Xueping Zhou;Yan Xie;Boli Hu;Jianxiang Wu
关键词:
-
Identification of Interaction Between Geminivirus and Plant Proteins by Bimolecular Fluorescence Complementation (BiFC)
作者:Min Du;Xueping Zhou;Xiuling Yang
关键词:Bimolecular fluorescence complementation;Confocal microscopy;Geminivirus;Protein-protein interaction;Subcellular localization