数字农科院2.0

AP2X-8 Is Important for Tachyzoite Growth and Bradyzoite Differentiation of Toxoplasma gondii

文献类型： 外文期刊

作者： Li Xiu Sun;Meng Wang;Hany M. Elsheikha;Shi Chen Xie;Bao Quan Fu;Xing Quan Zhu;Guo Hua Liu

作者机构：

关键词： AP2 transcription factors;AP2X-8;bradyzoite differentiation;tachyzoite growth;Toxoplasma gondii;toxoplasmosis

期刊名称： Animals

ISSN： 2076-2615

年卷期： 2025 年 15 卷 9 期

页码：

收录情况： SCIE（2025版）

摘要： Toxoplasma gondii has a complex life cycle involving three main stages: oocysts (containing sporozoites), tachyzoites, and tissue cysts (containing bradyzoites). Understanding the mechanisms that regulate the transition between tachyzoites and bradyzoites has been a major focus of T. gondii research. Previous studies have identified several AP2 transcription factors, such as AP2IV-3, AP2IX-9 and AP2IX-4, as important regulators of this process. In this study, we investigated the role of another AP2 factor, AP2X-8. Using C-terminal endogenous tagging combined with immunofluorescence analysis, we determined that AP2X-8 is a nuclear protein constitutively expressed in both tachyzoite and bradyzoite stages. We then constructed an ap2X-8 knockout strain using CRISPR-Cas9-mediated homologous recombination. Through a series of in vitro phenotypic assays, including plaque formation, invasion, replication, egress, and bradyzoite differentiation, we assessed the impact of ap2X-8 deletion. Our results demonstrated that AP2X-8 promotes tachyzoite growth and suppresses the bradyzoite differentiation in vitro. These findings provide new insights into the regulatory role of AP2X-8 in T. gondii development and lay the groundwork for future investigations into parasite differentiation mechanisms.

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