数字农科院2.0

Identification of two novel T cell epitopes on the E2 protein of classical swine fever virus C-strain

文献类型： 外文期刊

作者： Xiaotian Zhao;Xiao Wang;Mengqi Yuan;Xin Zhang;Xiaoke Yang;Xiangyu Guan;Shuwen Li;Jifei Ma;Hua Ji Qiu;Yongfeng Li

作者机构：

关键词： Classical swine fever virus;E2 protein;Homodimers;Inhibition;T cell epitopes

期刊名称： Veterinary Microbiology

ISSN： 0378-1135

年卷期： 2023 年 284 卷

页码：

收录情况： SCIE（2023版）

摘要： C-strain, also known as the HCLV strain, is a well-known live attenuated vaccine against classical swine fever (CSF), a devastating disease caused by classical swine fever virus (CSFV). Vaccination with C-strain induces a rapid onset of protection, which is associated with virus-specific gamma interferon (IFN-γ)-secreting CD8+ T cell responses. The E2 protein of CSFV is a major protective antigen. However, the T cell epitopes on the E2 protein remain largely unknown. In this study, eight overlapping nonapeptides of the E2 protein were predicted and synthesized to screen for potential T cell epitopes on the CSFV C-strain E2 protein. Molecular docking was performed on the candidate epitopes with the swine leukocyte antigen-1*0401. The analysis obtained two highly conserved T cell epitopes, 90STEEMGDDF98 and 331ATDRHSDYF339, which were further identified by enzyme-linked immunospot assay. Interestingly, the mutants deleting or substituting the epitopes are nonviable. Further analysis demonstrated that 90STEEMGDDF98 is crucial for the E2 homodimerization, while CSFV infection is significantly inhibited by the 331ATDRHSDYF339 peptide treatment. The two novel T cell epitopes can be used to design new vaccines that are able to provide rapid-onset protection.

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