数字农科院2.0

Effect of GnRH Active Immunisation on Reproductive Performance of Male Sprague Dawley Rats

文献类型: 外文期刊

作者: Cheng Qi Zhang;Shuang Shuang Li;Bo Hu;Li Wen Xu;Jia Jia Liu;Ya Jie Sun;Xue Bai

作者机构:

关键词: active immunisation;GnRH;rats;testosterone

期刊名称: International Journal of Molecular Sciences

ISSN: 1661-6596

年卷期: 2024 年 25 卷 6 期

页码:

收录情况: SCIE(2024版)

摘要: To investigate the effect of active immunisation with gonadotropin-releasing hormone (GnRH) on the reproductive function in male Sprague Dawley (SD) rats, 24 42-day-old rats were randomly assigned to treatment with GnRH6-MAP, GnRH-OVA, a surgical castration group, and a blank control group. Each rat in the treatment groups was intramuscularly injected at 6, 8, and 10 weeks of age. The serum concentrations of testosterone (T), follicle-stimulating hormone (FSH), luteinising hormone (LH), and anti-GnRH antibodies were determined using enzyme-linked immunosorbent assays. The results showed that active immunisation with recombinant GnRH6-MBP and GnRH-OVA significantly increased the serum levels of anti-GnRH antibodies and reduced the serum concentrations of testosterone compared to the black control. Eight weeks after immunisation, the rats’ testes were surgically removed for morphological evaluation, showing atrophy of the convoluted vasculature, relative emptying of the lumen, and insignificant differentiation of spermatogonial cells, which were increased in weight and volume compared with the blank control group. These findings indicated that active immunisation with GnRH can lead to testicular atrophy and reduce gonadal hormone concentrations, suggesting that GnRH is a highly effective immunogen.

分类号:

  • 相关文献

[1]GnIH/GnRH对鸟类排卵的调控作用. 张蕊,常玲玲,穆春宇,付胜勇,汤青萍,卜柱. 2018

[2]RFRP-3对哺乳动物生殖功能和能量平衡的影响. 向伟,赖平,张宝云,王凭青,储明星,樊奇,刘重旭,谭颖. 2012

[3]GnRH在青年摩杂一代水牛下丘脑-垂体-卵巢轴的免疫组化定位. 凌泽继,黎金秀,秦津,潘堂峰,宋小白,杨柄壮,许典新,韩涛,潘琼,李瑞明,谢莹雪,周辰瑜. 2009

[4]布氏田鼠GnRH基因克隆及不同组织和发育阶段的基因表达特征. 刘岚,陈燕,李宁,王大伟,任飞,宋英,郭聪,刘晓辉. 2017

[5]GnRH主动免疫对雄性藏绵羊血液中甲状腺激素含量的影响. 宋天增,韩兴发,谭遥,刘亚成,曹晓涵,杜小刚,孟风艳,储明星,曾宪垠. 2016

[6]GnRH主动免疫雌性大鼠调控机制研究. 曹晓涵,韩兴发,杜小刚,陈志渝,孟风艳,储明星,曾宪垠. 2016

[7]GnRH主动免疫对雄性藏绵羊血液中甲状腺激素含量的影响. 周玉芹,宋天增,韩兴发,谭遥,刘亚成,曹晓涵,杜小刚,孟风艳,储明星,曾宪垠. 2017

[8]SD雄鼠3种不同新蛋白构型GnRH去势抗原免疫学及生物学效果对比研究. 韩兴发,李俊丽,曹晓涵,储明星,曾宪垠. 2019

[9]PCB118对GT1-7细胞GnRH分泌及Kisspeptin/GPR54信号通路蛋白表达的影响. 黄苑,苏晓鸥,张维,齐香荣,林刚. 2019

[10]Physiological interactions between the hypothalamic-pituitary-gonadal axis and spleen in rams actively immunized against GnRH. Ren, Xiaohua,Zhou, Yuqin,Cao, Xiaohan,Du, Xiaogang,Meng, Fengyan,Tan, Yao,Liu, Yacheng,Zeng, Xianyin,Zeng, Yu,Song, TianZeng,Feng, Jing,Chu, Mingxing.

[11]Identification Of Prolificacy-Related Differentially Expressed P.roteins From Sheep (Ovis A ries) Hypothalamus By Comparative Proteomics. Zhang, ZB, Tang, JS, Di, R, Liu, QY, Wang, XY, Gan, SQ, Zhang, XS, Zhang, JL, Chen, W, Hu, WP, Chu, MX. 2019

[12]Ovariectomy induces abdominal fat accumulation by improving gonadotropin-releasing hormone secretion in mouse. Yongli Wang,Yidong Wang,Li Liu,Huanxian Cui. 2022

[13]Lin28b-let-7 Modulates mRNA Expression of GnRH1 Through Multiple Signaling Pathways Related to Glycolysis in GT1-7 Cells. Yujing Xie,Xin Li,Meng Wang,Mingxing Chu,Guiling Cao. 2025

[14]Advances in Timed Artificial Insemination: Integrating Omics Technologies for Enhanced Reproductive Efficiency in Dairy Cattle. Jesse Oluwaseun Ayantoye,Hubdar Ali Kolachi,Xiaomeng Zhang,Muhammad Shahzad,Omaima Mohamed Tawfik Kandil,Pengcheng Wan,Xueming Zhao. 2025

[15]Protective Effect of Selenium on Aflatoxin B1-Induced Testicular Toxicity in Mice. Cao, Zheng,Shao, Bing,Xu, Feibo,Liu, Yunfeng,Li, Yanfei,Zhu, Yanzhu.

[16]Effect of testosterone and melatonin on social dominance and agonistic behavior in male Tscheskia triton. Zhang, Jianxu,Zhang, Zhibin,Wang, Dawei.

[17]Early immunocastration of male pigs with Improvac (R) - Effect on boar taint, hormones and reproductive organs. Brunius, Carl,Zamaratskaia, Galia,Lundstrom, Kerstin,Andersson, Kristina,Chen, Gang,Norrby, Mattias,Madej, Andrzej. 2011

[18]Changes of plasma growth hormone, insulin-like growth factors-I, thyroid hormones, and testosterone concentrations in embryos and broiler chickens incubated under monochromatic green light. Zhang, Lin,Wu, Shugeng,Wang, Jing,Qiao, Xuan,Yue, Hongyuan,Zhang, Haijun,Qi, Guanghai,Zhang, Lin,Yao, Junhu. 2014

[19]Fecal hormone variation during prolonged social interaction in male Tscheskia triton. Wang, Zhenlong,Zhang, Jianxu,Zhang, Zhibin,Wang, Dawei,Wang, Zhenlong,Wang, Dawei,Wang, Zhenlong,Zhang, Jianjun,Zhang, Jianjun. 2009

[20]α-Linolenic acid-regulated testosterone biosynthesis via activation of the JNK-SF-1 signaling pathway in primary rooster Leydig cells. Zhi Xian Zhao,Ming Yu Shang,Cheng Long,Xue Jun Yao,Xiao Bo Gao,Yong Guo,Xi Hui Sheng,Xiang Guo Wang,Kai Xing,Long Fei Xiao,Xiao Long Qi. 2023

作者其他论文 更多>>