文献类型: 外文期刊
作者: Cheng Qi Zhang;Shuang Shuang Li;Bo Hu;Li Wen Xu;Jia Jia Liu;Ya Jie Sun;Xue Bai
作者机构:
关键词: active immunisation;GnRH;rats;testosterone
期刊名称: International Journal of Molecular Sciences
ISSN: 1661-6596
年卷期: 2024 年 25 卷 6 期
页码:
收录情况: SCIE(2024版)
摘要: To investigate the effect of active immunisation with gonadotropin-releasing hormone (GnRH) on the reproductive function in male Sprague Dawley (SD) rats, 24 42-day-old rats were randomly assigned to treatment with GnRH6-MAP, GnRH-OVA, a surgical castration group, and a blank control group. Each rat in the treatment groups was intramuscularly injected at 6, 8, and 10 weeks of age. The serum concentrations of testosterone (T), follicle-stimulating hormone (FSH), luteinising hormone (LH), and anti-GnRH antibodies were determined using enzyme-linked immunosorbent assays. The results showed that active immunisation with recombinant GnRH6-MBP and GnRH-OVA significantly increased the serum levels of anti-GnRH antibodies and reduced the serum concentrations of testosterone compared to the black control. Eight weeks after immunisation, the rats’ testes were surgically removed for morphological evaluation, showing atrophy of the convoluted vasculature, relative emptying of the lumen, and insignificant differentiation of spermatogonial cells, which were increased in weight and volume compared with the blank control group. These findings indicated that active immunisation with GnRH can lead to testicular atrophy and reduce gonadal hormone concentrations, suggesting that GnRH is a highly effective immunogen.
分类号:
- 相关文献
作者其他论文 更多>>
-
Insights into potential flavor-active peptides and taste-related compounds in Longjing teas: A comparative study of ‘Longjing 43’ and ‘Qunti’ cultivars
作者:Kangni Yan;Jiatong Wang;Mengxue Zhou;Qunhua Peng;Abdelkader Bassiony Mahmoud;Xue Bai;Susanne Baldermann;Xinbing Jiang;Shan Feng;Yan Wu;Jianyu Fu;Zhi Lin;Haipeng Lv;Jiang Shi
关键词:Flava-3-ols;Flavor-active peptides;Longjing tea;Maillard peptides;Peptide-T1R1 complex;Umami taste
-
The Discovery of Potential Repellent Compounds for Zeugodacus cucuribitae (Coquillett) from Six Non-Favored Hosts
作者:Yu Fu;Yupeng Chen;Yani Wang;Xinyi Fu;Shunda Jin;Chunyan Yi;Xue Bai;Youqing Lu;Wang Miao;Xingyu Geng;Xianli Lu;Rihui Yan;Zhongshi Zhou;Fengqin Cao
关键词:insect repellent;molecular docking;network pharmacology;olfactory genes;Zeugodacus cucuribitae (Coquillett)
-
Driving Factors of Rural Land-Use Change from a Multi-Scale Perspective: A Case Study of the Loess Plateau in China
作者:Bo Hu;Qingsong Ni;Zongfeng Chen;Xueqi Liu;Pingan Liu;Ziyi Yuan
关键词:influencing factors;Loess plateau;multi-scale;rural land-use/cover change;spatiotemporal pattern
-
Recombinant canine distemper virus expressing virus-like particle VP2 protein of mink enteritis virus protects minks against lethal challenges of both viruses
作者:Yuan Jiang;Yiyang Sun;Boyu Zhai;Tianjie Chen;Yang Li;Lina Ren;Yuchen Ma;Kun Han;Jianke Wang;Zhenwei Bi;Bo Hu;Jianjun Zhao
关键词:Bivalent live vaccine;Canine distemper virus;Capsid protein 2;Mink;Mink enteritis virus;Virus-like particles
-
Dissection of genetic basis underlying heat stress response of Apis cerana
作者:Ao Mei Li;Chun Xiu Pang;Xiao Lin Cai;Fu Chang Zhuo;Bo Hu;Xue Hua Huang;Jia Xiang Huang;Yu Ming Lu
关键词:Antioxidant activity;Apis cerana;DEGs;Heat stress;Transcriptomic
-
Integrated proteomics and metabolomics elucidate HSD17B12 regulation of intramuscular fat deposition for enhanced beef quality
作者:Yuan Liu;Shuang Liu;Xue Feng;Mengli Yang;Xue Bai;Dawei Wei;Bei Cai;Runjun Yang;Lupei Zhang;Yun Ma
关键词:DIA-PRM;Lipid metabolism;Longissimus dorsi;Muscle quality
-
Preparation of Monoclonal Antibodies Against the gD Protein of Feline Herpesvirus Type-1 by mRNA Immunization
作者:Chengqi Zhang;Yawen Liu;Guangrong Zhao;Bo Hu;Liwen Xu;Jiajia Liu;Yajie Sun;Xiaolan Guo;Xiaoyu Deng;Shizhen Lian;Tiyun Han;Mengwei Xu;Shi Xu;Xue Bai
关键词:clinical diagnosis;feline herpesvirus type-1;gD protein;monoclonal antibody;mRNA vaccine