Hen Egg White Lysozyme (HEWL) confers resistance to Verticillium wilt in cotton by inhibiting the spread of fungus and generating ROS burst
文献类型: 外文期刊
作者: 郭文芳;李刚强;王楠;杨彩峰;彭华康;王梦琪;刘德虎
关键词: Hen egg white lysozyme; antifungal protein; transgenic cotton; disease resistance; Verticillium wilt; ROS burst
期刊名称: International Journal of Molecular Sciences
ISSN: 1661-6596
年卷期: 2023 年
页码:
收录情况: SCIE(2023版)
摘要: Verticillium wilt is a soil-borne vascular disease caused by the fungal pathogen Verticillium dahliae. It causes great harm to upland cotton (Gossypium hirsutum) yield and quality. A previous study has shown that Hen egg white lysozyme (HEWL) exerts strong inhibitory activity against V. dahliae in vitro. In the current study, we introduced the HEWL gene into cotton through the Agrobacterium-mediated transformation, and the exogenous HEWL protein was successfully expressed in cotton. Our study revealed that HEWL was able to significantly inhibit the proliferation of V. dahlia in cotton. Consequently, the overexpression of HEWL effectively improved the resistance to Verticillium wilt in transgenic cotton. In addition, ROS accumulation and NO content increased rapidly after the V. dahliae inoculation of plant leaves overexpressing HEWL. In addition, the expression of the PR genes was significantly up-regulated. Taken together, our results suggest that HEWL significantly improves resistance to Verticillium wilt by inhibiting the growth of pathogenic fungus, triggering ROS burst, and activating PR genes expression in cotton.
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