数字农科院2.0

Expression Profiling Of Bombyx Mori G.loverin2 Gene And Its S ynergistic Antifungal Effect With Cecropin A Against Beauveria Bassiana

文献类型： 外文期刊

作者： Lu, Dingding;Lu, Dingding;Hou, Chengxiang;Qin, Guangxing;Qin, Guangxing;Lu, Dingding;Hou, Chengxiang;Geng, Tao;Gao, Kun;Gao, Kun;Guo, Xijie;Guo, Xijie;Geng, Tao

作者机构：

关键词： Bombyx mori; Bmgloverin2; BmCecA; Beauveria bassiana; Synergistic antifungal activity

期刊名称： GENE

ISSN： 0378-1119

年卷期： 2017 年 600 卷

页码：

摘要： Gloverin2 is a cationic and glycine-rich antimicrobial peptide whose expression can be induced in fat body of silkworm (Bombyx mod) larvae exposed to bacteria. The purpose of this study is to identify the roles of Bombyx mod gloverin2 (Bmgloverin2) during entomopathogenic fungus Beauveria bassiana infection. Fluorescent quantitative real-time PCR analysis indicated that the relative expression level of Bmgloverin2 gene was up-regulated in the silkworm larvae infected by B. bassiana. The cDNA of Bmgloverin2 was cloned from the silkworm by RT-PCR and the DNA segment of the Bmgloverin2 peptide (without signal peptide sequence) was inserted into pCzn1 expression plasmid and expressed in E. coli ArcticExpress (DE3). SOS-PAGE results revealed that soluble recombinant Bmgloverin2 was successfully expressed and purified. Polyclonal antibody against the Bmgloverin2 was successfully produced with the expressed recombinant protein. Western blot analysis indicated that Bmgloverin2 could be detected in the fat body of silkworm larvae infected with B. bassiana, suggesting that the expression of Bmgloverin2 could be induced by B. bassiana infection in silkworm. Antifungal assays indicated that the Bmgloverin2 had a synergistic antifungal activity with B. mod cecropin A (BmCecA) to entomopathogenic fungus B. bassiana both in vitro and in vivo in the silkworm larvae. This is the first report that Bmgloverin2 exhibits synergistic effect with BmCecA in antifungal activity against B. bassiana. The study demonstrates that Bmgloverin2 is an antifungal protein which plays an important role in synergistic antifungal activity with other antimicrobial peptide in silkworm. (C) 2016 Elsevier B.V. All rights reserved.

分类号：

  • 相关文献

[1]Molecular Cloning And Characterization Of H.igh Mobility Group Box ( Hmgb) Gene From Beauveria Bassiana- Infected Silkworm, Bombyx Mori. Ruilin, L.,Han, W.,Ruilin, L.,Xijie, G.,Xijie, G.,Han, W.,Chengxiang, H.,Chengxiang, H.,Chengxiang, H.,Dingding, L.,Dingding, L.. 2017

[2]An Aldo-Keto Reductase, Bbakr1, Is I.nvolved In Stress Response A nd Detoxification Of Heavy Metal Chromium But Not Required For Virulence In The Insect Fungal Pathogen, Beauveria Bassiana. Lu, Zhuoyue,Zhao, Xin,Luo, Tingying,Luo, Linli,Li, Min,He, Zhangjiang,Zhang, Yongjun,Wang, Huifang. 2018

[3]The Beauveria Bassiana Gas3 Beta-Glucanosyltransferase C.ontributes To Fungal Adaptation T o Extreme Alkaline Conditions. Bai, Yuting,Bai, Yuting,Chen, Qiyan,Chen, Qiyan,Keyhani, Nemat O.,Liu, Pengfei,Liu, Pengfei,Zhang, Tongbing,Luo, Zhibing,Luo, Zhibing,Luo, Zhibing,Luo, Zhibing,Zhang, Tongbing,Zhang, Yongjun,Zhang, Yongjun. 2018

[4]Entomopathogenicity Of Three Muscardine Fungi, B.eauveria Bassiana, Isaria Fumosorosea A nd Metarhizium Anisopliae, Against The Asian Citrus Psyllid, Diaphorina Citri Kuwayama (Hemiptera: Psyllidae). Majeed, M. Z.,Fiaz, M.,Majeed, M. Z.,Majeed, M. Z.,Afzal, M.,Majeed, M. Z.,Ma, Chun-Sen. 2017

[5]Biocontrol Potential of Some Entomopathogenic Fungal Strains Against Bean Aphid Megoura japonica (Matsumura). Duy Nam Trinh,Duy Nam Trinh,Duy Nam Trinh,Duy Nam Trinh,Qiu, Dewen,Thi Kim Lien Ha. 2020

[6]In vitro pathogenicity of the fungi Beauveria bassiana and Lecanicillium lecanii at different temperatures against the whitefly, Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae). Gadhi, Muswar Ali,Sokea, Tum,Qiu, Dewen,Abdulle, Yusuf Ali,Keerio, Azhar Uddin,Nazir, Talha,Jatoi, Ghulam Hussain,Anwar, Tauqir,Jatoi, Ghulam Hussain,Keerio, Azhar Uddin. 2020

[7]Functional characterization of four antimicrobial defensins in the globally invasive pest Tuta (Phthorimaea) absoluta (Lepidoptera: Gelechiidae). Bo Xu, Guohui Zhang, Nannan Zhu, Jörg Romeis, Jana Collatz, Guifen Zhang, Cong Huang, Yibo Zhang, Fanghao Wan. 2025

[8]Functional Analysis Of A Mirna-Like Small Rna Derived From Bombyx Mori Cytoplasmic Polyhedrosis Virus. Guo, Xi-Jie,Guo, Xi-Jie,Geng, Tao,Jiang, Xiao-Xu,Chen, Tian,Hou, Cheng-Xiang,Hou, Cheng-Xiang,Gao, Kun,Pan, Zhong-Hua,Gao, Kun,Guo, Jian-Yong,Guo, Jian-Yong,Wang, Yong-Sheng,Shang, Meng-Ke,Wang, Yong-Sheng,Wu, Ping,Wu, Ping. 2020

[9]Inhibition Of Heat Shock Protein 9.0 Suppresses Bombyx Mori N ucleopolyhedrovirus Replication In B. Mori. Guo, X. J.,Huang, H. L.,Zhang, S. L.,Tang, X. D.,Guo, X. J.,Wu, P.,Tang, X. D.,Shang, Q.,Wu, P.,Wu, P.. 2019

[10]Expression Analysis And Functional Identification O.f Several Genes Related T o Diapause In Bombyx Mori. Tang, Shunming,Tang, Shunming,Tan, Zhichen,Shen, Xingjia,Qian, Ping,Shen, Xingjia,Chen, Yanhua,Jiang, Tao,Qian, Ping,Li, Jiashuang,Jiang, Tao. 2019

[11]Expression Profile Of Several Genes O.n Ecdysteroidogenic Pathway Related T o Diapause In Pupal Stage Of Bombyx Mori Bivoltine Strain. Tang, Shunming,Zhao, Qiaoling,Huang, Jinshan,Zhu, Juan,Zhao, Qiaoling,Huang, Jinshan,Tang, Shunming,Chen, Yanrong,Shen, Xingjia,Shen, Xingjia. 2019

[12]Transcriptome Sequencing Reveals Potential Mechanisms O.f Diapause Preparation In B ivoltine Silkworm Bombyx Mori (Lepidoptera: Bombycidae). Shen, Xing-jia,Chen, Yan-hua,Jiang, Tao,Shen, Xing-jia,Tan, Zhi-cheng,Chen, Yan-hua,Chen, Yan-rong,Chen, Yan-rong,Hao, Bi-fang,Xie, Yu-chen,Chen, Yan-rong,Jiang, Tao,Tan, Zhi-cheng,Zhu, Juan,Tang, Shun-ming,Xie, Yu-chen,Tang, Shun-ming,Wang, Sheng-peng,Hao, Bi-fang,Huang, Jin-shan,Zhu, Juan,Wang, Sheng-peng,Huang, Jin-shan. 2017

[13]Expressional Analysis Of The Silkworm S.torage Protein 1 And I dentification Of Its Interacting Proteins. Gao, Kun,Geng, Tao,Li, Ruilin,Hou, Chengxiang,Lv, Dingding,Hou, Chengxiang,Guo, Xijie,Guo, Xijie,Hu, Congwu,Hu, Congwu,Li, Ruilin,Gao, Kun. 2019

[14]Silkworm Storage Protein Bm30K-19G1 Has A. Certain Antifungal Effects O n Beauveria Bassiana. Hou, Chengxiang,Hu, Congwu,Hou, Chengxiang,Hu, Congwu,Li, Ruilin,Lv, Dingding,Li, Ruilin,Gao, Kun,Shi, Yali,Guo, Xijie,Geng, Tao,Shi, Yali,Gao, Kun,Guo, Xijie. 2019

[15]Dna Methylomes And Transcriptomes Analysis R.eveal Implication Of Host D na Methylation Machinery In Bmnpv Proliferation In Bombyx Mori. Zhang, Shaolun,Shang, Qi,Zhong, Jinbo,Guo, Xijie,Yin, Haotong,W, Ping,Hou, Qirui,Hou, Qirui,Guo, Xijie,Huang, Haoling,W, Ping,Hou, Qirui,W, Ping. 2019

[16]Comparative Proteomic Analysis Reveals Immune Competence In Hemolymph Of Bombyx Mori Pupa Parasitized By Silkworm Maggot Exorista Sorbillans. Xu, An-Ying,Gao, Li,Li, Gang,Qian, He-Ying,Zhang, Mei-Rong,Qian, He-Ying,Xu, Ping-Zhen,Wu, Yang-Chun,Xu, An-Ying,Li, Gang,Xu, Ping-Zhen,Zhang, Mei-Rong,Wu, Yang-Chun. 2019

[17]Expression Analysis Of Mrna Decay Of Maternal Genes During Bombyx Mori Maternal-To-Zygotic Transition. Pang, Huilin,Chen, Tao,Zhang, Guozheng,Xu, Pingzhen,Zhang, Guozheng,Zhang, Meirong,Chen, Tao,Xu, Pingzhen,Zhang, Meirong. 2019

[18]Knockdown Of Bmtcp-1 Beta Delays B.mnpv Infection In Vitro. Wang, Xue-yang,Shao, Zuo-min,Chen, Qian-ying,Sun, Xia,Sun, Xia,Wang, Xue-yang,Wu, Yang-chun,Xu, Zhen-ping,Xu, Zhen-ping,Wu, Yang-chun,Li, Mu-wang,Li, Mu-wang,Xu, Jia-ping. 2019

[19]Identification and characterization of a novel endoglucanase (CMCase) isolated from the larval gut of Bombyx mori. Ma, Ru-Jian,Wang, Chun-Yan,Liu, Yan-Wei,Sivakumar, Thasma Raman,Ren, Zi-Xu,Fang, Ying,Jia, Jun-Qiang,Gui, Zhong-Zheng,Jia, Jun-Qiang,Gui, Zhong-Zheng. 2014

[20]Computational identification and characteristics of novel microRNAs from the silkworm (Bombyx mori L.). Huang, Yong,Tang, Shun Ming,Wang, Li Gang,Shen, Xing Jia,Zou, Quan,Huang, Yong,Tang, Shun Ming,Wang, Li Gang,Shen, Xing Jia. 2010

作者其他论文 更多>>