数字农科院2.0

A Double-Label Time-Resolved Fluorescent Strip F.or Rapidly Quantitative Detection O f Carbofuran Residues In Agro-Products

文献类型： 外文期刊

作者： Zhang, Q; Qu, QY; Chen, SS; Liu, XW; Li, PW

作者机构：

关键词： Double-label; Time-resolved fluorescent immunochromatographic assay (TRFICA); N-Methylcarbamate pesticide; Carbofuran; Residue; Agro-products

期刊名称： FOOD CHEMISTRY

ISSN： 0308-8146

年卷期： 2017 年 231 卷

页码：

收录情况： JCR（2021版） ; EI（2021版）

摘要： A rapid and quantitative time-resolved fluorescent immunochromatographic assay (TRFICA) for detecting carbofuran residues in agro-products was reported in this paper. This assay was developed based on double-label immunoprobes, one of which was a carbofuran-specific antibody coupled with europium microbeads for the test (T) line signal while the other was mouse IgG coupled with europium microbeads for the control (C) line signal. Quantitative relationships between carbofuran concentrations and T/C ratios were established to determine the analyte concentration. To increase assay accuracy, four standard curves were established for the agro-products (green bean, cabbage, apple, and pear). The limits of detection (LODs) ranged from 0.04 to 0.76 mg L-1. The spiked recoveries of carbofuran in the agro-products were in the range of 81-103%, which was in good agreement with a standard HPLC method. Therefore, we provided a new and reliable method for determination of N-methylcarbamate pesticide carbofuran residues in agro-products including vegetables and fruits. (C) 2017 Published by Elsevier Ltd.

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