数字农科院2.0

Recombinant Mtb9.8 Of Mycobacterium Bovis S.timulates Tnf-Alpha And Il-1 B eta Secretion By Raw264.7 Macrophages Through Activation Of Nf-Kappa B Pathway Via Tlr2

文献类型： 外文期刊

作者： Liu, SQ; Jia, H; Hou, SH; Xin, T; Guo, XY; Zhang, GM; Gao, XT; Li, M; Zhu, WY; Zhu, HF

作者机构：

期刊名称： SCIENTIFIC REPORTS

ISSN： 2045-2322

年卷期： 2018 年 8 卷

页码：

收录情况： JCR（2021版）

摘要： The Mtb9.8 antigenic protein of Mycobacterium bovis/Mycobacterium tuberculosis has been identified as a target of the T-cell response. However, the interaction of Mtb9.8 with Toll-like receptors (TLRs) and the relevant signaling pathways have not been fully clarified. In this study, recombinant Mtb9.8 (rMtb9.8) derived from M. bovis-stimulated RAW264.7 cells initiated the secretion of TNF-alpha and IL-1 beta in a dose-dependent manner. Blocking assays show that TLR2-neutralizing antibody decreases the production of TNF-alpha and IL-1 beta. Moreover, NF-kappa B activation is associated with TNF-alpha and IL-1 beta production by rMtb9.8 stimulation, and rMtb9.8 stimulation also induces the phosphorylation of NF-kappa B p65 at Ser536 and its rapid nuclear translocation in RAW264.7 cells. Furthermore, NF-kappa B luciferase activity is rapidly activated in response to rMtb9.8 in RAW264.7 cells and is also significantly increased in rMtb9.8-induced HEK293-TLR2. However, these activations were abrogated in cells with a dominantnegative mutation of NF-kappa B p65 and by treatment with anti-TLR2 antibody. We also find that rMtb9.8 induces the activation of IRF-1. These findings indicate that M. bovis-derived rMtb9.8 activates the NF-kappa B pathway via TLR2 in RAW264.7 cells. In particular, it phosphorylates NF-kappa B p65 at Ser536 and induces nuclear translocation, thereby leading to the production of TNF-alpha and IL-1 beta, which correlates with the induction of IRF-1.

分类号：

  • 相关文献
作者其他论文 更多>>