数字农科院2.0

Trans10, Cis12-Conjugated Linoleic Acid Exhibits A. Stronger Antioxidant Capacity T han Cis9, Trans11-Conjugated Linoleic Acid In Primary Cultures Of Laying Hen Hepatocytes

文献类型： 外文期刊

作者： Qi, XL; Wang, J; Yue, HY; Wu, SG; Zhang, YN; Ni, HM; Guo, Y; Zhang, HJ; Qi, GH

作者机构：

关键词： conjugated linoleic acid; hepatocyte; Nrf2; oxidative injury; laying hen

期刊名称： POULTRY SCIENCE

ISSN： 0032-5791

年卷期： 2018 年 97 卷 12 期

页码：

收录情况： JCR（2021版）

摘要： The objective of this study consisting of 2 trials was to investigate the antioxidant role of conjugated linoleic acid (CLA) isomers (c9, t11-CLA and t10, c12-CLA) and the underlying mechanism by which they act in modulating redox status in a primary laying hen hepatocyte culture. In trial 1, the cytotoxicity of CLA isomers or linoleic acid (LA) (0, 25, 50, 100, 200, 400, 800 mu mol/L) was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The concentration of CLA isomers or LA (25, 50, 100 mu mol/L) for proper antioxidant activity was evaluated by measuring the antioxidant enzyme activity. In trial 2, there were 5 groups: control group, cells were untreated; H2O2 group, cells were exposed to 4 mmol/L H2O2 for 2 h; c9, t11 or t10, c12 or LA group, cells were treated with c9, t11-CLA or t10, c12-CLA or LA for 24 h and then exposed to 4 mmol/L H2O2 for 2 h. Trial 1 showed that the non-toxic dose range for CLA isomers was 0 to 200 mu mol/L. The optimum concentration of c9, t11-CLA and t10, c12-CLA for trial 2 was 100 mu mol/L. In trial 2, pretreatment with t10, c12-CLA but not c9, t11-CLA attenuated the increase in reactive oxygen species (ROS) compared to hydrogen peroxide (H2O2) group (P < 0.05). t10, c12-CLA elevated the superoxide dismutase (SOD) and catalase (CAT) activities compared with the H2O2 group (P < 0.05). In addition, t10, c12-CLA up-regulated the mRNA expression of nuclear factor E2-related factor-2 (Nrf2) as well as its target genes, Cu-Zn superoxide dismutase (SOD1) and CAT (P < 0.05). Pretreatment with t10, c12-CLA but not c9, t11-CLA decreased Nrf2 protein expression in the cytoplasm and increased Nrf2 protein expression in the nucleus compared with the H2O2 group (P < 0.05). The results indicate that t10, c12-CLA exhibits a stronger antioxidant capacity than c9, t11-CLA in primary cultured laying hen hepatocytes. t10, c12-CLA increases the activity and mRNA expression of antioxidant enzymes via facilitating nuclear translocation of Nrf2.

分类号：

  • 相关文献

[1]Conjugated Linoleic Acid Regulates Lipid M.etabolism Through The Expression O f Selected Hepatic Genes In Laying Hens. Wang, SH, Wang, WW, Zhang, HJ, Wang, J, Chen, Y, Wu, SG, Qi, GH. 2019

[2]A Novel Mechanism Of Gamma-Aminobutyric A.cid (Gaba) Protecting Human U mbilical Vein Endothelial Cells (Huvecs) Against H2O2-Induced Oxidative Injury. Peng, Yuande,Hu, Zhenxiu,Gong, Wenbing,Peng, Yuande,Gong, Wenbing,Hu, Zhenxiu,Xie, Chunliang,Xie, Chunliang,Zhu, Zuohua,Shi, Zhigang,Zhu, Zuohua. 2019

[3]Theaflavins Improve Insulin Sensitivity Through R.egulating Mitochondrial Biosynthesis In P almitic Acid-Induced Hepg2 Cells. Kang, Hyunuk,He, Puming,Kim, Eunhye,Wu, Jiafan,Kim, Eunhye,Kang, Hyunuk,Li, Bo,He, Puming,Wu, Yuanyuan,Wu, Yuanyuan,Li, Bo,Tong, Tuantuan,Soomi, Park,Soomi, Park,Guo, Na,Guo, Na,Ren, Ning,Wu, Jiafan,Tong, Tuantuan,Tu, Youying,Ren, Ning,Tu, Youying. 2018

[4]Graphene Oxide Quantum Dots As N.ovel Nanozymes For Alcohol I ntoxication. Sun, AQ, Mu, L, Hu, XG. 2017

[5]Nobiletin And Its Colonic Metabolites S.uppress Colitis-Associated Colon Carcinogenesis B y Down-Regulating Inos, Inducing Antioxidative Enzymes And Arresting Cell Cycle Progression. Wu, X, Song, MY, Gao, ZL, Sun, Y, Wang, MQ, Li, F, Zheng, JK, Xiao, H. 2017

[6]Immunomodulation effects of polyphenols from thinned peach treated by different drying methods on RAW264.7 cells through the NF-κB and Nrf2 pathways. Chongting Guo,Jinfeng Bi,Xuan Li,Jian Lyu,Xuan Liu,Xinye Wu,Jianing Liu. 2021

[7]The Nrf2 Pathway Is Required F.or Intracellular Replication Of T oxoplasma Gondii In Activated Macrophages. Pang, Y, Zhang, ZX, Chen, YT, Cao, SN, Yang, XQ, Jia, HL. 2019

[8]Acetaminophen-Induced Hepatocyte Injury: C2-Ceramide And O.ltipraz Intervention, Hepatocyte Nuclear F actor 1 And Glutathione S-Transferase A1 Changes. Chang, YC, Wang, F, Yang, Y, Zhang, YY, Muhammad, I, Li, R, Li, CW, Li, Y, Shi, CX, Ma, X, Hao, BL, Liu, FP. 2019

[9]Echinococcus multilocularis infection induces UBE2N suppression via exosomal emu-miR-4989. Cai, Mengting,Ding, Juntao,Li, Yating,He, Guitian,Yang, Jing,Liu, Tingli,Guo, Xiaola,Yang, Xing,Wang, Xiaoqiang,Cho, William C.,Harandi, Majid Fasihi,Zheng, Yadong. 2021

[10]Impact of region on the composition of milk fatty acids in China. Yang, Yongxin,Wang, Jiaqi,Yuan, Tingjie,Bu, Dengpan,Yang, Jinhui,Zhou, Lingyun,Sun, Peng,Zhang, Juanxia,Yang, Yongxin. 2013

[11]PRODUCTION OF CONJUGATED LINOLEIC ACID BY WHOLE-CELL OF LACTOBACILLUS PLANTARUM A6-1F. Zhao, Hong-wei,Lv, Jia-ping,Li, Shu-rong. 2011

[12]Production of conjugated linoleic acid by Propionibacterium freudenreichii. Wang, Li-Min,Lv, Jia-Ping,Chu, Zhong-Qiu,Cui, Yuan-Yuan,Ren, Xing-Huan. 2007

[13]Lipase-catalyzed acylation of l-carnitine with conjugated linoleic acid in [Bmim]PF6 ionic liquid. Tian, Jinqiang,Wang, Qiang,Tian, Jinqiang,Wang, Qiang,Zhang, Zhongyuan. 2009

[14]Effect of dietary conjugated linoleic acid (CLA) on the growth and lipid metabolism of geese and fatty acid composition of their tissues. Zhang, Xu-hui,Wang, Bao-wei,Wang, Lei,Long, Fang-yu,Yang, Zhi-gang,Yu, Shi-hao,Wang, Ya-chao,Wei, Xiao-xiao,Jing, Li-zhen,Liu, Guang-lei. 2008

[15]Effects of trans-10, cis-12-conjugated linoleic acid on mice are influenced by the dietary fat content and the degree of murine obesity. Li, Shi-Li,Li, Mei-Juan,Gou, Ke-Mian,Ma, Shu-Yu,Fan, Zhi-Ying,Xu, Bin-Rui,Cao, Wen-Guang.

[16]Effectiveness of linoleic and linolenic acid for enhancing conjugated linoleic acid in milk from dairy cows.. Dhiman, T. R.,Shijun, L.. 2006

[17]Influence of fish/soybean oil supplementation on milk conjugated linoleic acid and mammary gland SCD gene expression in dairy cows. Bu, D. P.,Wang, J. Q.,Dhiman, T. R.,Liu, S. J.. 2007

[18]Effectiveness of Oils Rich in Linoleic and Linolenic Acids to Enhance Conjugated Linoleic Acid in Milk from Dairy Cows. Bu, D. P.,Wang, J. Q.,Dhiman, T. R.,Liu, S. J..

[19]Effects of dietary conjugated linoleic acids on lipid metabolism and antioxidant capacity in laying hens. Qi, Xiaolong,Wu, Shugeng,Zhang, Haijun,Yue, Hongyuan,Xu, Shaohui,Ji, Feng,Qi, Guanghai.

[20]The effect of steam-flaked and extruded full-fat soybeans on the concentration of conjugated linoleic acid in the milk fat of dairy cows. Li, Yanling,Meng, Qingxiang,Li, Yanling,Diao, Qiyu. 2009

作者其他论文 更多>>