数字农科院2.0

Detection Of Glycan-Binding Proteins Using G.lycan-Functionalized Quantum Dots And G old Nanoparticles

文献类型： 外文期刊

作者： Zhang, ZX; Qi, XX; Chai, JF; Wu, PX; Lv, X; Cheng, SH; Li, XB

作者机构：

关键词： glycan-binding protein; concanavalin A; fluorescence resonance energy transfer; gold nanoparticles; quantum dots

期刊名称： JOURNAL OF CARBOHYDRATE CHEMISTRY

ISSN： 0732-8303

年卷期： 2018 年 37 卷 4 期

页码：

收录情况： JCR（2021版）

摘要： Many pathogens can use their glycan-binding proteins (GBPs) to interact with glycan receptors on host cell surface. Here, a novel strategy is presented for the detection of GBPs based on fluorescence resonance energy transfer (FRET). Using mannosefunctionalized quantum dots and mannose-functionalized gold nanoparticles, a FRET assay system was developed and a mannose-specific GBP concanavalin A could be detected in 5 min with a detection limit of 3 nM. This result indicated the potential for rapid and sensitive biosensing of various kinds of GBPs and pathogens by changing the glycan structure on the surface of QDs and AuNPs. [GRAPHICS] .

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