数字农科院2.0

B-Class Mads-Box Tm6 Is A C.andidate Gene For Tomato M ale Sterile-15(26)

文献类型： 外文期刊

作者： Cao, X; Liu, XY; Wang, XT; Yang, MX; van Giang, T; Wang, J; Liu, XL; Sun, S; Wei, K; Wang, XX; Gao, JC; Du, YC; Qin, Y; Guo, YM; Huang, ZJ

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期刊名称： THEORETICAL AND APPLIED GENETICS

ISSN： 0040-5752

年卷期： 2019 年 132 卷 7 期

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收录情况： JCR（2021版） ; EI（2021版）

摘要： Key messageTomato male sterile-15(26) locus was fine-mapped to an interval of 44.6kb, and a B-class MADS-box gene TM6 was identified as the candidate gene.AbstractMale sterile lines have been widely used for hybrid seed production in many crop plants. The tomato male sterile-15(26) (ms-15(26)) mutant displays abnormal stamens and exerted stigmas and is suitable for practical use. In this study, the ms-15(26) locus was fine-mapped to a 44.6kb interval that contained four putative genes. Thereinto, Solyc02g084630 encodes tomato B-class MADS-box gene TM6 (syn. TDR6), which plays an important role in stamen development. Sequencing revealed that there was a 12.7kb deletion in the ms-15(26) region, where the promoter and first four exons of the TM6 gene were absent. ms-15(47), an allele of ms-15(26), also contained the same deletion in the TM6 gene. And the other allele ms-15 mutant contained a single-nucleotide polymorphism (SNP, C to A) in the coding region of the TM6 gene, which led to a missense mutation (G to W). The codominant insertion/deletion (InDel) marker MS26D and codominant derived cleaved amplified polymorphic sequence (dCAPS) marker MS15C were developed based on the deletion and SNP, respectively. A real-time quantitative reverse-transcription PCR showed that expression of the TM6 gene was barely detectable in the flowers of the ms-15(26) and ms-15(47) mutants. In addition, other floral organ identity genes, pollen development marker genes, and pistil marker genes were differentially expressed between wild type and mutant flowers. These findings may facilitate functional analysis of the TM6 gene and help in the marker-assisted selection of ms-15 and its alleles in tomatobreeding.

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