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Optimization Of Protective Agents And F.reeze-Drying Conditions For Lactobacillus C asei Lt-L614

文献类型： 外文期刊

作者： Xie, YiPeng;Weng, Mengling;You, Qianqian;XinLi;Wang, Lijun;Wang, Lijun;Weng, Mengling;Wang, Lijun;Bin Feng;ZhiguangTang;Wang, Lijun;Juan Peng;GanlinChen;GanlinChen

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关键词： Lactobacillus casei LT-L614; Vacuum Freeze Drying; Freeze-Dried Protective Agent; Gastrointestinal Simulation

期刊名称： JOURNAL OF BIOBASED MATERIALS AND BIOENERGY

ISSN： 1556-6560

年卷期： 2019 年 13 卷 1 期

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摘要： In order to improve the way of preserving probiotics so that it will be easier to transfer and preserve, this study focus on developing optimum conditions and protective agents for the freeze-drying of Lactobacillus casei LT-L614. By means of a L-9(3(4)) orthogonal test, the optimum formula for the protective agents was found as follows: 100 g/L skim milk, 60 g/L mannitol, 15 g/L L-tyrosine, 50 g/L Tween-80. Besides, the optimal freeze-drying conditions were also investigated by taking three factors into consideration and results were: ratio of bacterial solution and protection agent was 1:7.5, pre-freezing time was 5 h and 10% skimmed milk was used for rehydration. The optimal viable cell count was 9.80 x 10(11) cfu/g under these conditions. The results also indicated that the freeze-dried cells have a much higher survival rate compared to the untreated cells. Finally, the tolerance of both the synbiotics and non-lyophilized cells in simulated gastrointestinal fluid was also test. Although the survival rate of the bioconjugate decreased by only 5.37% compared with the non-lyophilized cells. The number of live cells in the digestive juice remained to be 3.27 x 10(9) cfu/mL.

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