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Cultivation Of Lactobacillus Casei Lt-L614 I.n High Density With S ugarcane Molasses: Optimization Of Processing

文献类型： 外文期刊

作者： You, Qianqian;Feng, Bin;Tang, Zhiguang;Wang, Lijun;Weng, Mengling;Weng, Mengling;Chen, Zhineng;Wang, Lijun;Peng, Juan;Wang, Lijun;Weng, Mengling;Wang, Lijun;Lie, Yonghong;Weng, Mengling;Li, Xin;Chen, Jian

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关键词： Sugarcane Molasses; Lactobacillus casei LT-L614; High Density Culture; Logistic Equations

期刊名称： JOURNAL OF BIOBASED MATERIALS AND BIOENERGY

ISSN： 1556-6560

年卷期： 2019 年 13 卷 6 期

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摘要： The culture medium was optimized based on the MRS medium by single factor tests and response surface technology. Specifically, the recipe was as follows: molasses of 80 g/L, soybean peptone of 10 g/L, yeast powder of 15 g/L, corn juice of 70 mL/L, sodium citrate of 8.5 g/L. The culture conditions were also optimized as initial pH of 6.9, temperature of 37 degrees C, and inoculation quantity of 2%. The combination of neutralization with intermittent feeding was applied for the fermentation. Moreover, a dynamic model was proposed for the cell growth of Lactobacillus casei LT-L614, through fitting reducing sugar amount and fermentation time. The feeding time interval was optimized according to the logistic model. It resulted in an optimal viable count of 9 x 12 x 10(11) cfu/mL, which was increased by two orders for magnitude compared to that without optimization.

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