数字农科院2.0

High-Throughput Identification Of Micrornas In Taenia Hydatigena, A Cestode Threatening Livestock Breeding Industry

文献类型： 外文期刊

作者： Wu, JE; Yang, J; He, GT; Guo, XL; Kutyrev, I; Kandil, OM; Zheng, YD

作者机构：

关键词： Taenia Hydatigena; Mirna; Cestode

期刊名称： INFECTION GENETICS AND EVOLUTION

ISSN： 1567-1348

年卷期： 2019 年 75 卷

页码：

收录情况： JCR（2021版）

摘要： Infection of Cysticercus tenuicollis, the larval stage of Taenia hydatigena, is extensively found in sheep and pigs and jeopardizes the breeding and meat industry. miRNAs are a subclass of small noncoding regulatory RNAs and closely associated with the pathogenesis and biology of parasites. Here, using HiSeq sequencing we identified 49 known and 2 potential novel miRNAs in C. tenuicollis, of which both thy-miR-71 and -87 were predominant. Using RT-qPCR, 6 selected miRNAs were validated, and thy-miR-71 and -miR-87 were confirmed to be highly expressed, with the copy number of approximately 82,340 +/- 2079 and 19,580 +/- 609 per 1 ng total RNA, respectively. Similar to other cestodes, T. hydatigena was predicted to have two conserved miRNA clusters thymiR-71/2c/2b and thy-miR-4989/277, and three members of the former were confirmed to reside sequentially within the genomic region of 253 bp by PCR. The current data provide us a valuable resource for further studies of a role of miRNAs in T. hydatigena biology and infection.

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