数字农科院2.0

Mapk-Directed Activation Of The Whitefly Transcription Factor Creb Leads To P450-Mediated Imidacloprid Resistance

文献类型： 外文期刊

作者： Yang, X; Deng, S; Wei, XG; Yang, J; Zhao, QN; Yin, C; Du, TH; Guo, ZJ; Xia, JX; Yang, ZZ; Xie, W; Wang, SL; Wu, QJ; Yang, FS; Zhou, XG; Nauen, R; Bass, C; Zhang, YJ

作者机构：

关键词： Cytochrome P450; Creb; Mapk; Neonicotinoid Insecticide; Insecticide Resistance

期刊名称： PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

ISSN： 0027-8424

年卷期： 2020 年 117 卷 19 期

页码：

收录情况： JCR（2021版）

摘要： The evolution of insect resistance to pesticides poses a continuing threat to agriculture and human health. While much is known about the proximate molecular and biochemical mechanisms that confer resistance, far less is known about the regulation of the specific genes/gene families involved, particularly by trans-acting factors such as signal-regulated transcription factors. Here we resolve in fine detail the trans-regulation of CYP6CM1, a cytochrome P450 that confers resistance to neonicotinoid insecticides in the whitefly Bemisia tabaci, by the mitogen-activated protein kinase (MAPK)-directed activation of the transcription factor cAMP-response element binding protein (CREB). Reporter gene assays were used to identify the putative promoter of CYP6CM1, but no consistent polymorphisms were observed in the promoter of a resistant strain of B. tabaci (imidacloprid-resistant, IMR), which over-expresses this gene, compared to a susceptible strain (imidacloprid-susceptible, IMS). Investigation of potential trans-acting factors using in vitro and in vivo assays demonstrated that the bZIP transcription factor CREB directly regulates CYP6CM1 expression by binding to a cAMP-response element (CRE)-like site in the promoter of this gene. CREB is overexpressed in the IMR strain, and inhibitor, luciferase, and RNA interference assays revealed that a signaling pathway of MAPKs mediates the activation of CREB, and thus the increased expression of CYP6CM1, by phosphorylation-mediated signal transduction. Collectively, these results provide mechanistic insights into the regulation of xenobiotic responses in insects and implicate both the MAPK-signaling pathway and a transcription factor in the development of pesticide resistance.

分类号：

  • 相关文献

[1]Mechanisms Of Resistance To Thiamethoxam A.nd Dinotefuran Compared To I midacloprid In The Brown Planthopper: Roles Of Cytochrome P450 Monooxygenase And A P450 Gene Cyp6Er1. Sun, Xiaoqin,Sun, Xiaoqin,Ali, Shahbaz,Hou, Maolin,Gong, Youhui,Ali, Shahbaz,Gong, Youhui,Hou, Maolin,Hou, Maolin. 2018

[2]Gene Expression Profiles Of The S.outhern House Mosquito Culex Q uinquefasciatus During Exposure To Permethrin. Reid, WR, Zhang, L, Gong, YH, Li, T, Liu, NN. 2018

[3]Critical Analysis of Multi-Omic Data from a Strain of Plutella xylostella Resistant to Bacillus thuringiensis Cry1Ac Toxin. Kang S., Zhu X., Wu Q., Wang S., Crickmore N., Zhang Y., Guo Z.. 2022

[4]Metal-Organic Framework Preparation Using Magnetic G.raphene Oxide-Beta-Cyclodextrin For Neonicotinoid P esticide Adsorption And Removal. Liu, GY, Li, LY, Xu, DH, Huang, XD, Xu, XM, Zheng, SN, Zhang, YG, Lin, HA. 2017

[5]Integrative Transcriptional And Metabolic Analyses P.rovide Insights Into Cold S pell Response Mechanisms In Young Shoots Of The Tea Plant. Hao, Xinyuan,Hao, Xinyuan,Tang, Hu,Tang, Hu,Wang, Lu,Zeng, Jianming,Wang, Bo,Wang, Lu,Wang, Xinchao,Wang, Xinchao,Yang, Yajun,Zeng, Jianming,Yue, Chuan,Yue, Chuan,Yue, Chuan,Yang, Yajun. 2018

[6]Aspirin Eugenol Ester Inhibits Agonist-Induced P.latelet Aggregation In Vitro B y Regulating Pi3K/Akt, Mapk And Sirt 1/Cd40L Pathways. Li, Jian-Yong,Dong-Shuai Shen. 2019

[7]The Antiviral Effects Of Harmine A.gainst Bohv-1 Infection In V itro. Jiang, XY, Zou, J, Zhuang, YC, Yuan, WF, Zhu, LQ, Zhu, GQ. 2017

[8]Key Cannabis Salt-Responsive Genes and Pathways Revealed by Comparative Transcriptome and Physiological Analyses of Contrasting Varieties. Zhang, Jiangjiang,Zhang, Cuiping,Huang, Siqi,Chang, Li,Li, Jianjun,Tang, Huijuan,Dey, Susmita,Biswas, Ashok,Du, Dengxiang,Li, Defang,Zhao, Lining. 2021

[9]Molecular characterization, expression and interaction of MAPK, MAPKK and MAPKKK genes in upland cotton. Yin Z.,Zhu W.,Zhang X.,Chen X.,Wang W.,Lin H.,Wang J.,Ye W.. 2021

[10]Ginsenoside Rg1 Ameliorates Chronic Social Defeat Stress-Induced Depressive-Like Behaviors And Hippocampal Neuroinflammation. Jiang, N, Lv, JW, Wang, HX, Huang, H, Wang, Q, Lu, C, Zeng, GR, Liu, XM. 2020

[11]14-3-3 Protein Of Neospora Caninum M.odulates Host Cell Innate I mmunity Through The Activation Of Mapk And Nf-Kappa B Pathways. Li, S, Gong, PT, Zhang, N, Li, X, Tai, LX, Wang, X, Yang, ZT, Yang, J, Zhu, XQ, Zhang, XC, Li, JH. 2019

[12]Evaluation Of Pre-Slaughter Low-Current/High-Frequency Electrical S.tunning On Lipid Oxidative S tability, Antioxidant Enzyme Activity And Gene Expression Of Mitogen-Activated Protein Kinase/Nuclear Factor Erythroid 2-Related Factor 2 (Mapk/Nrf2) Signalling Pathway In. Xu, L, Wang, J, Yue, HY, Farnell, MB, Yang, HM, Wang, ZY, Qi, GH. 2019

[13]Dietary Canolol Induces Apoptosis In H.uman Cervical Carcinoma Hela C ells Through Ros-Mapk Mediated Mitochondrial Signaling Pathway: In Vitro And In Vivo. Xia, XY, Xiang, X, Huang, FH, Zheng, MM, Zhang, Z, Han, L. 2019

[14]Mapk Mediates No/Cgmp-Induced Gaba Accumulation I.n Soybean Sprouts. Jiao, CF, Duan, YQ, Lin, Q. 2019

[15]Non-Tal Effectors From Xanthomonas Oryzae P.v. Oryzae Suppress Peptidoglycan-Triggered M apk Activation In Rice. Yang, Bing,Zhou, Junhui,Long, Juying,Song, Congfeng,Long, Juying,Yan, Fang,Yang, Bing,Song, Congfeng,Zhou, Huanbin,Yang, Bing. 2018

[16]Molecular characterization, expression and interaction of MAPK, MAPKK and MAPKKK genes in upland cotton. Yin Z.,Zhu W.,Zhang X.,Chen X.,Wang W.,Lin H.,Wang J.,Ye W.. 2021

[17]Lactobacillus Brevis 23017 Relieves Mercury T.oxicity In The Colon B y Modulation Of Oxidative Stress And Inflammation Through The Interplay Of Mapk And Nf-Kappa B Signaling Cascades. Jiang, XP, Gu, SS, Liu, D, Zhao, LL, Xia, S, He, XM, Chen, HY, Ge, JW. 2018

[18]Integrative Transcriptional And Metabolic Analyses Provide Insights Into Cold Spell Response Mechanisms In Young Shoots Of The Tea Plant. Hao, XY, Tang, H, Wang, B, Yue, C, Wang, L, Zeng, JM, Yang, YJ, Wang, XC. 2018

[19]Nadph-Cytochrome P450 Reductase Mediates The R.esistance Of Aphis (Toxoptera) C itricidus (Kirkaldy) To Abamectin. Ding, Bi-Yue,Jing, Tian-Xing,Jing, Tian-Xing,Wei, Dan-Dan,Ding, Bi-Yue,Tan, Yang,Wei, Dan-Dan,Wang, Jin-Jun,Wang, Jin-Jun,Dou, Wei,Dou, Wei,Tan, Yang. 2018

[20]Rna-Sequencing Of A Citrus Bud-Feeder, Podagricomela Weisei (Coleoptera: Chrysomelidae), Reveals Xenobiotic Metabolism/Core Rnai Machinery-Associated Genes And Conserved Mirnas. Wang, JJ,Peng, YY,Ye, C,Chang, TY,Yang, L,Niu, JZ,Shang, F,Ding, BY. 2019

作者其他论文 更多>>