数字农科院2.0

Single Virus Tracking With Quantum Dots Packaged Into Enveloped Viruses Using Crispr

文献类型： 外文期刊

作者： Yang, YB; Tang, YD; Hu, Y; Yu, F; Xiong, JY; Sun, MX; Lyu, C; Peng, JM; Tian, ZJ; Cai, XH; An, TQ

作者机构：

关键词： Single Virus Tracking; Quantum Dots; Clustered Regularly Interspaced Short Palindromic Repeats; Pseudorabies Virus; Viral Infection

期刊名称： NANO LETTERS

ISSN： 1530-6984

年卷期： 2020 年 20 卷 2 期

页码：

收录情况： JCR（2021版） ; EI（2021版）

摘要： Labeling viruses with high-photoluminescence quantum dots (QDs) for single virus tracking provides a visual tool to aid our understanding of viral infection mechanisms. However, efficiently labeling internal viral components without modifying the viral envelope and capsid remains a challenge, and existing strategies are not applicable to most viruses. Here, we have devised a strategy using the clustered regularly interspaced short palindromic repeats (CRISPR) imaging system to label the nucleic acids of Pseudorabies virus (PRV) with QDs. In this strategy, QDs were conjugated to viral nucleic acids with the help of nuclease-deactivated Cas9/gRNA complexes in the nuclei of living cells and then packaged into PRV during virion assembly. The processes of PRV-QD adsorption, cytoplasmic transport along microtubules, and nuclear entry were monitored in real time in both Vero and HeLa cells, demonstrating the utility and efficiency of the strategy in the study of viral infection.

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