数字农科院2.0

Fluorescence Immunoassay For Multiplex Detection Of Organophosphate Pesticides In Agro-Products Based On Signal Amplification Of Gold Nanoparticles And Oligonucleotides

文献类型： 外文期刊

作者： Zhang, C; Jiang, ZJ; Jin, MJ; Du, PF; Chen, G; Cui, XY; Zhang, YD; Qin, GX; Yan, FY; El-Aty, AMA; Hacimuftuoglu, A; Wang, J

作者机构：

关键词： Fluorescence Immunoassay; Organophosphate Pesticides; Oligonucleotide; Gold Nanoparticle

期刊名称： FOOD CHEMISTRY

ISSN： 0308-8146

年卷期： 2020 年 326 卷

页码：

收录情况： JCR（2021版） ; EI（2021版）

摘要： Herein, we developed a multi-analyte fluorescence immunoassay for detection of three organophosphate pesticides (triazophos, parathion, and chlorpyrifos) in various agro-products (rice, wheat, cucumber, cabbage, and apple) using fluorescently labeled oligonucleotide and gold nanoparticle (AuNP) signal amplification technology. The AuNP probes for the three analytes were constructed by simultaneously modifying the corresponding antibodies and fluorescently labeled oligonucleotides on the probe surface. Three fluorophores (6-FAM, Cy3, and Texas red) with high fluorescence intensity and little overlap of excitation/emission wavelengths were selected. The method showed satisfactory linearity for triazophos, parathion, and chlorpyrifos in the ranges of 0.01-20, 0.05-50, and 0.5-1000 mu g/L, respectively. For the 3 analytes, the limits of detection (LODs) were 0.007, 0.009, and 0.087 mu g/L, respectively. The average recoveries were 77.7-113.6%, with relative standard deviations (RSDs) of 7.1-17.1% in various food matrices. The proposed method offers great potential in food safety surveillance, and could be used as well as a reference for multi-residue analysis of other small-molecule contaminants.

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