数字农科院2.0

Identification, Characterization, And Functional Analysis Of Chitin Synthase Genes Inglyphodes Pyloaliswalker (Lepidoptera: Pyralidae)

文献类型： 外文期刊

作者： Shao, ZM; Li, YJC; Ding, JH; Liu, ZX; Zhang, XR; Wang, J; Sheng, S; Wu, FA

作者机构：

关键词： Glyphodes Pyloaliswalker; Chitin Synthase; Dfb; Rna Interference; Pest Control

期刊名称： INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

ISSN：

年卷期： 2020 年 21 卷 13 期

页码：

收录情况： JCR（2021版）

摘要： Glyphodes pyloalisWalker (G. pyloalis) causes significant damage to mulberry every year, and we currently lack effective and environmentally friendly ways to control the pest. Chitin synthase (CHS) is a critical regulatory enzyme related to chitin biosynthesis, which plays a vital role in the growth and development of insects. The function of CHS inG. pyloalis, however, has not been studied. In this study, two chitin synthase genes (GpCHSAandGpCHSB) were screened from our previously created transcriptome database. The complete coding sequences of the two genes are 5,955 bp and 5,896 bp, respectively. Expression ofGpCHSAandGpCHSBcould be detected throughout all developmental stages. Relatively high expression levels ofGpCHSAoccurred in the head and integument andGpCHSBwas most highly expressed in the midgut. Moreover, silencing ofGpCHSAandGpCHSBusing dsRNA reduced expression of downstream chitin metabolism pathway genes and resulted in abnormal development and wings stretching, but did not affect normal pupating of larvae. Furthermore, the inhibitor of chitin synthesis diflubenzuron (DFB) was used to further validate the RNAi result. DFB treatment significantly improved expression ofGpCHSA, exceptGpCHSB, and their downstream genes, and also effectedG. Pyloalimolting at 48 h (62% mortality rate) and 72 h (90% mortality rate), respectively. These results show thatGpCHSAandGpCHSBplay critical roles in the development and wing stretching inG. pyloalisadults, indicating that the genes are attractive potential pest control targets.

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