数字农科院2.0

Establishment of Eimeria tenella (local isolate) in chicken embryos

文献类型： 外文期刊

作者： Jiang, L.;Zhao, Q.;Zhu, S.;Han, H.;Dong, H.;Huang, B.

作者机构：

关键词： Eimeria tenella;chicken;embryo;in vitro cultivation;vaccine

期刊名称： PARASITE-JOURNAL DE LA SOCIETE FRANCAISE DE PARASITOLOGIE

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年卷期：

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收录情况： SCI

摘要： Development of an in vitro Eimeria (E.) tenella model could be valuable as a tool for vaccine, coccidiostats or molecular biology research. 1.0 x 104 sporozoites per 0.1 mL were inoculated into the allantoic cavity of ten-day-old chicken embryos. Thecomplete life-cycle of E. tenella was accomplished in eight-nine days at 37°C and 70 % humidity. The addition of 100 U insulin to the embryos could remarkably improve the output of oocysts. The development of the parasite within the embryos was systematically observed, allowing guidelines to be set regarding the appropriate times at which different developmental stages of the parasite may be sampled.

分类号： Q18

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