数字农科院2.0

An optimized, highly efficient, self-assembled, subvirus-like particle of infectious bursal disease virus (IBDV)

文献类型： 外文期刊

作者： Wang, Miao;Pan, Qing;Lu, Zhen;Li, Kai;Gao, Honglei;Qi, Xiaole;Gao, Yulong;Wang, Xiaomei;Wang, Xiaomei

作者机构：

关键词： Infectious bursal disease virus;Yeast;sVP

期刊名称： VACCINE

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Infectious bursal disease virus (IBDV) causes immunosuppression in young chickens, leading to increased susceptibility to other diseases and a reduction in the immune response to other vaccines. Thus, IBDV results in great economic losses to the poultry industry. The most effective method of prevention is vaccination. However, medium-virulence vaccines can cause bursal pathological damage and immunosuppression. Here, we describe a safer, self-assembled, subvirus-like particle (sVP) vaccine without a complex purification process. The IBD-VP2 gene was cloned into Pichia pastoris, and the expressed protein self-assembled into T=1 sVPs (similar to 23 nm). Immunization experiments showed that the sVP vaccine elicited high IBDV-neutralizing antibodies in each group, and all birds survived challenge with very virulent IBDV (vvIBDV). Additionally, IBDV RNA was not detected, and sterile immunity was achieved. In conclusion, the IBD-sVP is a suitable candidate for a recombinant subunit vaccine against IBDV. (C) 2016 Published by Elsevier Ltd.

分类号： R392

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