数字农科院2.0

Glycosylation Contributes to Thermostability and Proteolytic Resistance of rFIP-nha (Nectria haematococca)

文献类型: 外文期刊

作者: Yusi Liu;Tamara Hoppenbrouwers;Yulu Wang;Yingying Xie;Xue Wei;Haowen Zhang;Guoming Du;Khandader Md Sharif Uddin Imam;Harry Wichers;Zhen Li;Shanna Bastiaan-Net

作者机构:

关键词: digestion resistance;glycosylation;immunomodulatory proteins;structure;thermostability

期刊名称: Molecules

ISSN: 1420-3049

年卷期: 2023 年 28 卷 17 期

页码:

收录情况: SCIE(2023版)

摘要: Glycosylation is an important post-translational modification of proteins, contributing to protein function, stability and subcellular localization. Fungal immunomodulatory proteins (FIPs) are a group of small proteins with notable immunomodulatory activity, some of which are glycoproteins. In this study, the impact of glycosylation on the bioactivity and biochemical characteristics of FIP-nha (from Nectria haematococca) is described. Three rFIP-nha glycan mutants (N5A, N39A, N5+39A) were constructed and expressed in Pichia pastoris to study the functionality of the specific N-glycosylation on amino acid N5 and N39. Their protein characteristics, structure, stability and activity were tested. WT and mutants all formed tetramers, with no obvious difference in crystal structures. Their melting temperatures were 82.2 °C (WT), 81.4 °C (N5A), 80.7 °C (N39A) and 80.1 °C (N5+39A), indicating that glycosylation improves thermostability of rFIP-nha. Digestion assays showed that glycosylation on either site improved pepsin resistance, while 39N-glycosylation was important for trypsin resistance. Based on the 3D structure and analysis of enzyme cleavage sites, we conclude that glycosylation might interfere with hydrolysis via increasing steric hindrance. WT and mutants exerted similar bioactivity on tumor cell metabolism and red blood cells hemagglutination. Taken together, these findings indicate that glycosylation of FIP-nha impacts its thermostability and digestion resistance.

分类号:

  • 相关文献

[1]Linking the thermostability of FIP-nha (Nectria haematococca) to its structural properties. Liu, Yusi,Bastiaan-Net, Shanna,Zhang, Yuebin,Hoppenbrouwers, Tamara,Xie, Yingying,Wang, Yulu,Wei, Xue,Du, Guoming,Zhang, Haowen,Imam, Khandader M. D. Sharif Uddin,Wichers, Harry,Li, Zhen. 2022

[2]Current Understanding Of The Structure And Function Of Fungal Immunomodulatory Proteins. Liu, YS, Bastiaan-Net, S, Wichers, HJ. 2020

[3]N-glycosylation proteomic characterization and cross-species comparison of milk fat globule membrane proteins from mammals. Yang, Yongxin,Zheng, Nan,Zhao, Xiaowei,Zhang, Yangdong,Han, Rongwei,Ma, Lu,Zhao, Shengguo,Li, Songli,Guo, Tongjun,Zang, Changjiang,Wang, Jiaqi,Yang, Yongxin,Zhao, Xiaowei,Wang, Weiyu.

[4]Mechanistic understanding of N-glycosylation in Ebola virus glycoprotein maturation and function. Wang, Bin,Wang, Yujie,Yao, Xiaoyu,Hu, Dan,Zhang, Zhuo,Zheng, Yong-Hui,Liu, Chaonan,Zheng, Shimin,Xiang, Shi-Hua,Xiang, Shi-Hua,Zheng, Yong-Hui.

[5]Amino acid mutations in the env gp90 protein that modify N-linked glycosylation of the Chinese EIAV vaccine strain enhance resistance to neutralizing antibodies. Han, Xiue,Zhang, Ping,Yu, Wei,Li, Xiaodong,Xiang, Wenhua.

[6]N-glycosylation proteomic characterization and cross-species comparison of milk whey proteins from dairy animals. Yang, Yongxin,Zheng, Nan,Zhao, Xiaowei,Zhang, Yangdong,Han, Rongwei,Zhao, Shengguo,Yang, Jinhui,Li, Songli,Guo, Tongjun,Zang, Changjiang,Wang, Jiaqi,Yang, Yongxin,Zhao, Xiaowei,Han, Rongwei.

[7]Characterization of seed proteome in Brachypodium distachyon. Wang, Ke,Han, Xiaofeng,Dong, Kun,Gao, Liyan,Yan, Yueming,Wang, Ke,Han, Xiaofeng,Li, Hongyu,Ye, Xingguo,Ma, Wujun.

[8]Effect of glycosylation with xylose on the mechanical properties and water solubility of peanut protein films. Lin, Wei-Jing,Liu, Hong-Zhi,Shi, Ai-Min,Liu, Li,Wang, Qiang,Adhikari, Benu.

[9]Synthesis of alkyl beta-D-Glcp-(1 -> 3)-[beta-D-Glcp-(1 -> 6)-beta-D-Glcp-(1 -> 6)-beta-D-glucopyranosides with anti-tumor activity. Heng, Linsen,Zhao, Qianfei,Mei, Xiangdong,Lv, Haibin,Jiang, Xiaohui,Ning, Jun.

[10]Differential glycosylation of rhLf expressed in the mammary gland of transgenic mice. Zhao, CJ,Liu, ZL,Fan, BL,Dai, YP,Wang, LL,Zheng, M,Wang, ML,Niu, HL,Xi, FG,Li, N,Zhang, DL. 2006

[11]Development of a Rapid Immunochromatographic Lateral Flow Device Capable of Differentiating Phytase Expressed from Recombinant Aspergillus niger phyA2 and Genetically Modified Corn. Zhou, Xiaojin,Pu, Ling-Kui,Hui, Elizabeth,Zhang, Jun,Yu, Xiao-Lin,Tu, Zhiguan,Lin, Zhen,Zheng, Jian,Zhang, Jun,Liu, Oi,Liu, Oi,Zhane, Juan.

[12]An efficient synthesis of a dimer of the tetrasaccharide present in motif B of the Mycobacterium tuberculosis cell wall. Ning, J.

[13]Nontargeted Modification-Specific Metabolomics Investigation of Glycosylated Secondary Metabolites in Tea (Camellia sinensis L.) Based on Liquid Chromatography-High-Resolution Mass Spectrometry. Dai, Weidong,Tan, Junfeng,Xie, Dongchao,Li, Pengliang,Lv, Haipeng,Zhu, Yin,Guo, Li,Zhang, Yue,Peng, Qunhua,Lin, Zhi,Lu, Meiling.

[14]Glycosylation of the Hemagglutinin Protein of H5N1 Influenza Virus Increases Its Virulence in Mice by Exacerbating the Host Immune Response. Zhao, Dongming,Liang, Libin,Wang, Shuai,Li, Yanbing,Liu, Liling,Guan, Yuntao,Bu, Zhigao,Chen, Hualan,Zhao, Dongming,Nakao, Tomomi,Fukuyama, Satoshi,Bu, Zhigao,Kawaoka, Yoshihiro,Kawaoka, Yoshihiro.

[15]The Unique Glycosylation at Position 986 on the E2 Glycoprotein of Classical Swine Fever Virus Is Responsible for Viral Attenuation and Protection against Lethal Challenge. Yongfeng Li,Mengqi Yuan,Yuying Han,Libao Xie,Yuteng Ma,Su Li,Yuan Sun,Yuzi Luo,Weike Li,Hua Ji Qiu. 2022

[16]Exploring the diversity of plant proteome. Yanmei Chen,Yi Wang,Jun Yang,Wenbin Zhou,Shaojun Dai. 2021

[17]Glycosylation Analysis of Feline Small Intestine Following Toxoplasma gondii Infection. Zhai B.,Xie S.,Peng J.,Qiu Y.,Liu Y.,Zhu X.,He J.,Zhang J.. 2022

[18]Pectin supplementation ameliorates intestinal epithelial barrier function damage by modulating intestinal microbiota in lipopolysaccharide-challenged piglets. Wen X.,Zhong R.,Dang G.,Xia B.,Wu W.,Tang S.,Tang L.,Liu L.,Liu Z.,Chen L.,Zhang H.. 2022

[19]Glycosylated proteins with abnormal glycosylation changes are potential biomarkers for early diagnosis of breast cancer. Xinyu Chang,Uchechukwu Edna Obianwuna,Jing Wang,Haijun Zhang,Guanghai Qi,Kai Qiu,Shugeng Wu. 2023

[20]Stability of glycosylated complexes loaded with Epigallocatechin 3-gallate (EGCG). Jianyong Zhang,Hongchun Cui,Jiahuan Qiu,Xiaoqing Wang,Yixin Zhong,Caiping Yao,Lanying Yao,Qunxiong Zheng,Chun Hua Xiong. 2023

作者其他论文 更多>>