数字农科院2.0

Gene expression changes in chicken NLRC5 signal pathway associated with in vitro avian leukosis virus subgroup J infection

文献类型： 外文期刊

作者： Qiu, L. L.;Xu, L.;Guo, X. M.;Li, Z. T.;Wan, F.;Chen, G. H.;Chang, G. B.;Liu, X. P.

作者机构：

关键词： Avian leukosis virus;Chicken embryo fibroblast;NLRC5;Signal pathway;Real-time PCR

期刊名称： GENETICS AND MOLECULAR RESEARCH

ISSN： 1676-5680

年卷期： 2016 年 15 卷 1 期

页码：

收录情况： SCI

摘要： Nucleotide-binding oligomerization domain-like receptors (NLRs) play a key role in the innate immune response as pattern-recognition receptors. However, the role of NLRC5, which is a member of the NLR family, in NF-kappa B activation and MHC-I expression remains debatable. Infection with the J group avian leukosis virus (ALV-J) can result in immunosuppression and a subsequent increase in susceptibility to secondary infection. This results in huge economic losses to the poultry industry worldwide. Using quantitative real-time polymerase chain reaction (qRT-PCR), we investigated the mRNA expression levels of NLRC5 signal pathway-related genes in secondary chicken embryo fibroblasts 7 days after infection with ALV-J. The results indicated that, compared with the control groups, the expression levels of TLR7, MHC-I, and IL-18 increased significantly in the infected groups at 7 days post-infection (d.p.i.). The expression levels of NLRC5 and IL-6 were conspicuously downregulated at 7 d.p.i., but the expression levels of NF-kappa B, STAT1, and STAT3 were not significantly altered. These results suggest that NLRC5 and some genes involved in the NLRC5 pathway play a key role in antiviral immunity, typically the response to ALV-J infection. Moreover, MHC-I expression levels vary between different cell types.

分类号：

  • 相关文献

[1]REV感染对鸡NLRC5及其信号通路相关基因转录的影响. 刘向萍,仇玲玲,郭晓敏,王克华,陈国宏. 2016

[2]细菌内毒素对鸡NLRC5信号通路相关基因转录的影响. 仇玲玲,马腾,王洪志,徐璐,刘向萍,陈静,郭晓敏,李志腾,万方. 2015

[3]细菌内毒素对鸡NLRC5受体及IFN基因转录的影响. 常国斌,陈蓉,栾德琴,张颖,刘向萍,马腾,戴爱琴,周伟,陈国宏. 2011

[4]Transcriptome analysis of pre-immune state induced by interferon gamma inhibiting the replication of H9N2 avian influenza viruses in chicken embryo fibroblasts. Haozhi Song,Xingjian Liu,Xintao Gao,Jialei Li,Yuting Shang,Weisong Gao,Yinü Li,Zhifang Zhang. 2022

[5]Expression patterns of NLRC5 and key genes in the STAT1 pathway following infection with Salmonella pullorum. Qiu, Lingling,Ma, Teng,Chang, Guobin,Guo, Xiaomin,Xu, Lu,Zhang, Yang,Zhao, Wenming,Xu, Qi,Chen, Guohong,Liu, Xiangping.

[6]A mutation in the NLRC5 promoter limits NF-kappa B signaling after Salmonella Enteritidis infection in the spleen of young chickens. Chang, Guobin,Ma, Teng,Xu, Lu,Wang, Hongzhi,Li, Zhiteng,Guo, Xiaomin,Xu, Qi,Chen, Guohong,Liu, Xiangping.

[7]Coronavirus Porcine Deltacoronavirus Upregulates MHC Class I Expression through RIG-I/IRF1-Mediated NLRC5 Induction. Xiang Liu,Lingdan Yin,Mei Xue,Jianfei Chen,Liang Li,Fang Fu,Li Feng,Pinghuang Liu. 2022

[8]Identification of a linear B-cell epitope on the avian leukosis virus P27 protein using monoclonal antibodies. Li, Xiaofei,Qi, Xiaole,Wang, Yongqiang,Gao, Honglei,Gao, Yulong,Wang, Xiaomei,Li, Xiaofei,Qin, Liting,Sun, Yingjun,Cui, Xuezhi,Gao, Yadong,Zhu, Haibo.

[9]Avian leukosis virus subgroup A and B infection in wild birds of Northeast China. Li, Delong,Qin, Liting,Qi, Xiaole,Wang, Yongqiang,Wang, Xiaomei,Gao, Yulong,Li, Delong,Liu, Sidang,Gao, Honglei,Yang, Bo,Liu, Wansi,Zeng, Xiangwei.

[10]Development of an antigen-capture ELISA for the detection of avian leukosis virus p27 antigen. Yun, Bingling,Li, Delong,Zhu, Haibo,Liu, Wen,Qin, Liting,Liu, Zaisi,Wu, Guan,Wang, Yongqiang,Qi, Xiaole,Gao, Honglei,Wang, Xiaomei,Gao, Yulong. 2013

[11]Residues E53, L55, H59, and G70 of the cellular receptor protein Tva mediate cell binding and entry of the novel subgroup K avian leukosis virus. Xinyi Li,Yuntong Chen,Mengmeng Yu,Suyan Wang,Peng Liu,Lingzhai Meng,Ru Guo,Xiaoyan Feng,Mingxue Hu,Tana He,Xiaole Qi,Kai Li,Li Gao,Yanping Zhang,Changjun Liu,Hongyu Cui,Xiaomei Wang,Yulong Gao. 2023

[12]Development And Application Of A C.olloidal Gold Test Strip F or Detection Of Avian Leukosis Virus. Yu, MM, Bao, YL, Wang, MP, Zhu, HB, Wang, XY, Xing, LX, Chang, FF, Liu, YZ, Farooque, M, Wang, YQ, Qi, XL, Liu, CJ, Zhang, YP, Cui, HY, Li, K, Gao, L, Pan, Q, Wang, XM, Gao, YL. 2019

[13]Identification And Characterization Of A N.ovel Natural Recombinant Avian L eucosis Virus From Chinese Indigenous Chicken Flock. Liang, XY, Gu, YF, Chen, XY, Li, TF, Gao, YL, Wang, XM, Fang, C, Fang, SG, Yang, YY. 2019

[14]Functions and Application of the AP2/ERF Transcription Factor Family in Crop Improvement. Xu, Zhao-Shi,Chen, Ming,Li, Lian-Cheng,Ma, You-Zhi. 2011

[15]High mobility group box-1 regulates expression of EGFR, VEGF, StAR and TIMP1/2 in bovine granulosa cells through a mechanism involving TLR2/NF-κB. Yingying Xie,Kang Zhang,Jingyan Zhang,Kai Zhang,Xuequan Hu,Lei Wang,Xuezhi Wang,Jianxi Li. 2022

[16]Microbiome and Metabolic Changes of Milk in Response to Dietary Supplementation With Bamboo Leaf Extract in Dairy Cows. Zhan Jing-wei,Shen Yi-yuan,Li Xin,Zhang Hua,Niu Hui,Fang Luo-yun,Xiong Ben-hai,Tong Jin-jin,Jiang Lin-shu. 2021

[17]Hormone effects of eighteen bisphenol analogues and their effects on cellular homeostasis and the typical signal pathways. Mengmeng Ma,Dongmei Guo,Ruiguo Wang,Peilong Wang,Xiaoou Su. 2023

[18]Correlation of Coding and Non-Coding RNAs on the Fat Deposition of Yaks Under Different Feeding Systems. Xiong, Lin,Pei, Jie,Guo, Shaoke,Cao, Mengli,Ding, Zhiqiang,Kang, Yandong,Wu, Xiaoyun,Guo, Xian. 2025

[19]An ABF5b-HsfA2h/HsfC2a-NCED2b/POD4/HSP26 module integrates multiple signaling pathway to modulate heat stress tolerance in wheat. Wei, Ji-Tong,Zheng, Lei,Ma, Xiao-Jun,Yu, Tai-Fei,Gao, Xiang,Hou, Ze-Hao,Liu, Yong-Wei,Cao, Xin-You,Chen, Jun,Zhou, Yong-Bin,Chen, Ming,Jiang, Qi-Yan,Ma, You-Zhi,Zheng, Wei-Jun,Xu, Zhao-Shi. 2025

[20]铜胁迫对农田土壤酶活性、细菌和古菌数量的影响. 王斐,黄益宗,王小玲,高柱,余发新,徐峰,保琼莉,胡莹,乔敏. 2014

作者其他论文 更多>>