数字农科院2.0

Development of a recombinant OppA-based indirect hemagglutination test for the detection of antibodies against Haemophilus parasuis

文献类型： 外文期刊

作者： Chen, Shengli;Chu, Yuefeng;Zhao, Ping;He, Ying;Jian, Yingna;Liu, Yongsheng;Lu, Zhongxin

作者机构：

关键词： Haemophilus parasuis;OppA;Indirect hemagglutination test;Antibody detection

期刊名称： ACTA TROPICA

ISSN： 0001-706X

年卷期： 2015 年 148 卷

页码：

收录情况： SCI

摘要： An indirect hemagglutination (IHA) test that could detect antibodies against Haemophilus parasuis was developed. The full-length cDNA sequence of the oligopeptide permease ABC transporter membrane protein (OppA) gene was cloned, and inserted into the prokaryotic expression vector pET-30a(+) to construct recombinant plasmid pET-30a-OppA. The recombinant OppA protein was expressed partly in soluble form in Escherichia coil BL21 (DE3) and then purified by Ni2+ column. Furthermore, the recombinant OppA protein was used as an antigen to develop an IHA assay for detecting antibodies against H. parasuis. Results showed that this IHA test could detect species-specific antibodies against H. parasuis. Compared with currently available ELISA, the IHA test had a sensitivity of 85.0% and a specificity of 95.0%. The overall agreement between these two methods was 90.0%. The developed IHA test was used to evaluate the seroprevalence of H. parasuis in Hubei Province, China. The H. parasuis seroprevalence rate ranged from 5.5% to 26.2% in 325 tested clinical serum samples that were collected from three different pig farms in Hubei Province, China. The IHA test developed in this study will greatly contribute to the epidemiological surveys and immunization surveillance of H. parasuis. (C) 2015 Elsevier B.V. All rights reserved.

分类号：

  • 相关文献

[1]副猪嗜血杆菌单克隆抗体的制备及其B细胞抗原表位的初步鉴定. 郑楠,符芳,柴政,姜福成,王香玲,张雪云,王卓,李曦. 2013

[2]副猪嗜血杆菌单克隆抗体的制备及其B细胞抗原表位的鉴定. 杨玉菊,郑楠,符芳,柴政,姜福成,王香玲,张雪云,王卓,李曦. 2014

[3]Electrotransformation of Haemophilus parasuis with in vitro modified DNA based on a novel shuttle vector. Chen, Liping,Wu, Dongfang,Cai, Xuwang,Guo, Fengjuan,Xu, Xiaojuan,Chen, Huanchun,Chen, Liping,Blackall, P. J.. 2012

[4]Effects of nucleotide usage on the synonymous codon usage patterns of biofilm-associated genes in Haemophilus parasuis. Wang, L. Y.,Ma, L. N.,Liu, Y. S.. 2016

[5]Seroprevalence and risk factors associated with Haemophilus parasuis infection in Tibetan pigs in Tibet. Zhang, Nian-Zhang,Zhou, Dong-Hui,Huang, Si-Yang,Wang, Meng,Shi, Xin-Chun,Zhu, Xing-Quan,Shi, Xin-Chun,Ciren, Danba,Zhu, Xing-Quan. 2014

[6]Secondary Haemophilus parasuis infection enhances highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) infection-mediated inflammatory responses. Li, Jiangnan,Wang, Shengnan,Li, Changyao,Wang, Chunlai,Liu, Yonggang,Wang, Gang,He, Xijun,Hu, Liang,Liu, Yuanyuan,Cui, Mengmeng,Bi, Caihong,Shao, Zengyu,Cai, Xuehui,Huang, Li,Weng, Changjiang,Wang, Xiaojie,Xiong, Tao.

[7]Gene Expression Profiling of Cecropin B-Resistant Haemophilus parasuis. Wang, Chunmei,Chen, Fangzhou,Hu, Han,Li, Wentao,Chen, Pin,Liu, Yingyu,Ku, Xugang,He, Qigai,Chen, Huanchun,Wang, Chunmei,Xue, Feiqun,Wang, Yang.

[8]Deletion of HAPS_2096 Increases Sensitivity to Cecropin B in Haemophilus parasuis. Chen, Fanjie,Hu, Han,Li, Zhonghua,Huang, Jiacheng,Cai, Xuwang,Wang, Chunmei,He, Qigai,Cao, Jiyue,Wang, Chunmei.

[9]Identification of novel Haemophilus parasuis serovar 5 vaccine candidates using an immunoproteomic approach. Li, Gang,Xie, Fang,Li, Jianjun,Liu, Jiao,Li, Dapeng,Zhang, Yanhe,Liu, Siguo,Wang, Chunlai,Langford, Paul R.,Li, Yanwen.

[10]Loop-mediated isothermal amplification for the rapid detection of Haemophilus parasuis. Chen, Hao-Tai,Chu, Yue-Feng,Liu, Yong-Sheng,Zhang, Jie,Lu, Zhong-Xin. 2010

[11]Development of a neutralizing mouse-pig chimeric antibody with therapeutic potential against Haemophilus parasuis in Pichia pastoris. Chai, Zheng,Fu, Fang,Jiang, Fucheng,Tian, Huabin,Wang, Zhuo,Zheng, Nan,Zhang, Xueyun,Wang, Xiangling,Li, Xi.

[12]Multilocus sequence typing and virulence analysis of Haemophilus parasuis strains isolated in five provinces of China. Wang, Liyan,Ma, Lina,Liu, Yongan,Gao, Pengcheng,Li, Youquan,Li, Xuerui,Liu, Yongsheng.

[13]Identification Of Novel Haemophilus Parasuis S.erovar 5 Vaccine Candidates U sing An Immunoproteomic Approach. Li, G, Xie, F, Li, JJ, Liu, J, Li, DP, Zhang, YH, Langford, PR, Li, YW, Liu, S, Wang, CL. 2017

[14]Evaluation Of Glutathione-Binding Protein A O.f Haemophilus Parasuis As A Vaccine Candidate In A Mouse Model. Zhang, YH, Li, G, Xie, F, Liu, SG, Wang, CL. 2017

[15]Inhibition of Haemophilus parasuis by berberine and proteomic studies of its mechanism of action. Yue Jia,Changqi Hao,Qin Yang,Wanjiang Zhang,Gang Li,Siguo Liu,Xin Hua. 2021

[16]Serovars, Antimicrobial Susceptibility And Molecular Characteristics Of Haemophilus Parasuis Isolates In Southern China. Peng, L, Yuan, XQ, Fang, R, Liu, WZF, Wen, Q, Yang, XF. 2020

[17]Detection of anti-Trichinella antibodies in serum of experimentally-infected swine by immunochromatographic strip. Fu, B. Q.,Li, W. H.,Gai, W. Y.,Yao, J. X.,Qu, Z. G.,Xie, Z. Z.,Wang, Y. H.,Zhang, D. L.,Blaga, R.. 2013

[18]Development and clinical evaluation of a new gold-immunochromatographic assay for the detection of antibodies against field strains of pseudorabies virus. Guo, Dian-lei,Pan, Qi-wei,Li, Kun-peng,Li, Jun-qing,Shen, Han-wei,Wang, Xiang-ling,Zhang, Xun-yun,Li, Xue-song,Fu, Fang,Feng, Li,Li, Xi.

[19]Establishment and application of a solid-phase blocking ELISA method for detection of antibodies against classical swine fever virus. Yuying Cao,Li Yuan,Shunli Yang,Youjun Shang,Bin Yang,Zhizhong Jing,Huichen Guo,Shuanghui Yin. 2022

[20]Development and Evaluation of a Competitive Enzyme-Linked Immunosorbent Assay Based on Swine Monoclonal Antibodies for Detecting Neutralizing Antibodies against Senecavirus A. Ma, Xueqing,Huang, Jiaxin,Li, Kun,Ding, Kailu,Fu, Yuanfang,Zhang, Jing,Zhao, Zhixun,Li, Pinghua,Bai, Xingwen,Li, Dong,Liu, Xia,Zeng, Qiaoying,Liu, Zaixin,Sun, Pu,Lu, Zengjun. 2023

作者其他论文 更多>>