数字农科院2.0

Comparison of the structural characterization and biological activity of acidic polysaccharides from Cordyceps militaris cultured with different media

文献类型： 外文期刊

作者： Wu, Fengyao;Yan, Hui;Ma, Xiaoning;Jia, Junqiang;Zhang, Guozheng;Guo, Xijie;Gui, Zhongzheng;Jia, Junqiang;Zhang, Guozheng;Guo, Xijie;Gui, Zhongzheng

作者机构：

关键词： Cordyceps militaris;Media;Polysaccharide;Structure;Antioxidant;Splenocyte proliferation;In vitro

期刊名称： WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

ISSN： 0959-3993

年卷期： 2012 年 28 卷 5 期

页码：

收录情况： SCI

摘要： Two acidic polysaccharide fractions, CM-jd-CPS2 and CM-jd(Y)-CPS2, were isolated from the fruiting bodies of cultured Cordyceps militaris grown on solid rice medium and silkworm pupa, respectively, by hot-water extraction, ethanol precipitation and fractionation using ion-exchange column (DEAE-cellulose-52) and gel-filtration column (Sephadex G-100) chromatography. Their structural characterizations were performed by gas chromatography and fourier-transform infrared spectroscopy. Some differences existed between their structures, which indicated that culture media could influence the structure of polysaccharides of C. militaris. The antioxidant activities of CM-jd-CPS2 and CM-jd(Y)-CPS2 were evaluated by various methods in vitro. They had strong 2,2-diphenyl-1-picrylhydrazyl radical-scavenging activity and ferrous ion-chelating capacity, but moderate reducing power. The antioxidant activities of CM-jd(Y)-CPS2 were slightly higher than those of CM-jd-CPS2. These two acidic fractions were evaluated for proliferation of mouse splenocyte activity in vitro. They both possessed does-dependent mitogenic effects on mouse splenocytes, and could synergistically promote murine T- and B-lymphocytes induced by Con A and LPS. CM-jd(Y)-CPS2 exhibited stronger stimulatory activities upon immunomodulation than CM-jd-CPS2. These results are beneficial for the interpretation of the connection between polysaccharide structures and their biological activities.

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