数字农科院2.0

Distribution of puroindoline alleles in bread wheat cultivars of the Yellow and Huai valley of China and discovery of a novel puroindoline a allele without PINA protein

文献类型： 外文期刊

作者： Chen, F.;Zhang, F-Y.;Dong, Z-D.;Cui, D-Q.;Xia, X-C.

作者机构：

关键词： Bread wheat;Kernel hardness;Puroindoline allele;PINA-null alleles;Primer walking strategy

期刊名称： MOLECULAR BREEDING

ISSN： 1380-3743

年卷期： 2012 年 29 卷 2 期

页码：

收录情况： SCI

摘要： Kernel hardness is one of the most important factors determining the milling and processing quality of bread wheat (Triticum aestivum L.). In the present study, 267 wheat cultivars and advanced lines from the Yellow and Huai Valley of China, CIMMYT, Russia and Ukraine were used for identification of SKCS (Single Kernel Characterization System) hardness and puroindoline alleles. Results indicated that Pinb-D1b is the most popular genotype in wheat cultivars from the Yellow and Huai Valley, Russia and Ukraine, whereas PINA null is a predominant genotype in wheat cultivars and advanced lines from CIMMYT. Molecular characterization of PINA-null alleles indicated that one Chinese landrace Chiyacao had the allele Pina-D1l with a single nucleotide C deletion at position 265 in Pina coding region based on sequencing results, and 35 of 39 PINA-null alleles belonged to Pina-D1b according to PCR amplification with the sequence-tagged site (STS) marker Pina-N developed previously. The remaining three cultivars (Jiangdongmen, Heshangtou and Hongquanmang from China) with PINA-null alleles were characterized at the DNA level by a primer walking strategy, and the results showed that all three cultivars with PINA-null alleles possessed a uniform 10,415-bp deletion from -5,117 bp to +5,298 bp (ATG codon references zero), designated as Pina-D1r. Correspondingly, an STS marker Pina-N2 with an expected fragment size of 436-bp spanning the 10,415-bp deletion was developed for detection of the Pina-D1r allele. This study provided a useful molecular marker for straightforward detection of one of the PINA-null alleles and would also be helpful to further understand the molecular and genetic basis of kernel hardness in bread wheat.

分类号：

  • 相关文献

[1]Prevalence of a novel puroindoline b allele in Yunnan endemic wheats (Triticum aestivum ssp. yunnanense King). Chen, Feng,Yu, Yaxiong,Xia, Xianchun,He, Zhonghu. 2007

[2]A new puroindoline b mutation present in Chinese winter wheat cultivar Jingdong 11. Chen, F,He, ZH,Xia, XC,Lillemo, M,Morris, C. 2005

[3]Segregation analysis indicates that Puroindoline b-2 variants 2 and 3 are allelic in Triticum aestivum and that a revision to Puroindoline b-2 gene symbolization is indicated. Geng, Hongwei,Geng, Hongwei,Beecher, Brian S.,Morris, Craig F.,Pumphrey, Michael,He, Zhonghu,He, Zhonghu. 2013

[4]Physical mapping of puroindoline b-2 genes and molecular characterization of a novel variant in durum wheat (Triticum turgidum L.). Chen, F.,Xu, H. -X.,Zhang, F. -Y.,Dong, Z. -D.,Zhan, K. -H.,Cheng, X. -Y.,Cui, D. -Q.,Xia, X. -C.,He, Z. -H.,He, Z. -H.,Wang, D. -W.. 2011

[5]Identification of novel alleles induced by EMS-mutagenesis in key genes of kernel hardness and starch biosynthesis in wheat by TILLING. Li, Wenjie,Zhao, Baocun,Li, Wenjie,Guo, Huijun,Xie, Yongdun,Zhao, Linshu,Gu, Jiayu,Zhao, Shirong,Liu, Luxiang,Wang, Yongbin,Wang, Guangjin.

[6]Genotypic Variation in Wheat Flour Lysophospholipids. Liu, Lei,Guo, Qi,Waters, Daniel L. E.,Raymond, Carolyn A.,King, Graham J.,Guo, Qi,He, Zhonghu,Xia, Xianchun,He, Zhonghu.

[7]Molecular and biochemical characterization of puroindoline a and b alleles in Chinese landraces and historical cultivars. Chen, F,He, ZH,Xia, XC,Xia, LQ,Zhang, XY,Lillemo, M,Morris, CF. 2006

[8]Molecular characterization of the Puroindoline a-D1b allele and development of an STS marker in wheat (Triticum aestivum L.). Chen, Feng,Zhang, Fuyan,Cui, Dangqun,Morris, Craig,He, Zhonghu,Xia, Xianchun,He, Zhonghu. 2010

[9]Silencing of puroindoline a alters the kernel texture in transgenic bread wheat. He, Zhonghu,Xia, Lanqin,Geng, Hongwei,Chen, Xinmin,He, Zhonghu,Lillemo, Morten,Morris, Craig F.. 2008

[10]Seedling and slow rusting resistance to stripe rust in Chinese common wheats. Xia, X. C.,Zhou, X. C.,Niu, Y. C.,He, Z. H.,Zhang, Y.,Li, G. Q.,Wan, A. M.,Wang, D. S.,Chen, X. M.,Lu, Q. L.,Singh, R. P.. 2006

[11]Pattern analysis on protein properties of Chinese and CIMMYT spring wheat cultivars sown in China and CIMMYT. Zhang, Yong,He, Zhonghu,Zhang, Aimin,van Ginkel, Maarten,Pena, Roberto J.,Ye, Guoyou. 2006

[12]Milling quality and protein properties of autumn-sown Chinese wheats evaluated through multi-location trials. Zhang, Y,Zhang, Y,He, ZH,Ye, GY. 2005

[13]Characterization of A- and B-type starch granules in Chinese wheat cultivars. Zhang Yan,Guo Qi,Feng Nan,He Zhong-hu,Wang Jin-rong,Wang Shu-jun,He Zhong-hu. 2016

[14]Characterization of Chinese wheat germplasm for resistance to Fusarium head blight at CIMMYT, Mexico. He, Xinyao,Singh, Pawan K.,Schlang, Norbert,Duveiller, Etienne,Dreisigacker, Susanne,Payne, Thomas,He, Xinyao,He, Xinyao. 2014

[15]Characterization of the rich haplotypes of Viviparous-1A in Chinese wheats and development of a novel sequence-tagged site marker for pre-harvest sprouting resistance. Yang, Y.,Liu, S. X.,Sun, Y. Q.,Meng, J. Y.,Zhang, C. L.,Xia, L. Q.,Zhang, C. L.. 2014

[16]Pan bread and dry white Chinense qualities in Chinese winter wheats. He, ZH,Yang, J,Zhang, Y,Quail, KJ,Pena, RJ. 2004

[17]Genetic improvement of grain yield and associated traits in the southern China winter wheat region: 1949 to 2000. Zhu, H. Z.,Cai, S. B.,He, Z. H.,Zhang, X. K.,Xia, X. C.,Zhang, G. S.. 2007

[18]Pattern analysis on grain yield performance of Chinese and CIMMYT spring wheat cultivars sown in China and CIMMYT. Zhang, Y,He, ZH,Zhang, AM,van Ginkel, M,Ye, GY. 2006

[19]Characterization of two 1D-encoded omega-gliadin subunits closely related to dough strength and pan bread-making quality in common wheat (Triticum aestivum L.). Wang, Aili,Gao, Liyan,Li, Xiaohui,Zhang, Yanzhen,Yan, Yueming,He, Zhonghu,Xia, Xianchun,Zhang, Yong,He, Zhonghu. 2008

[20]Rapid genome evolution revealed by comparative sequence analysis of orthologous regions from four triticeae genomes. Gu, YQ,Coleman-Derr, D,Kong, XY,Anderson, OD.

作者其他论文 更多>>