数字农科院2.0

Structural characterization and antioxidant activity of purified polysaccharide from cultured Cordyceps militaris

文献类型： 外文期刊

作者： Wu, Fengyao;Yan, Hui;Ma, Xiaoning;Jia, Junqing;Zhang, Guozheng;Guo, Xijie;Gui, Zhongzheng;Jia, Junqing;Zhang, Guozheng;Guo, Xijie;Gui, Zhongzheng

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期刊名称： AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH

ISSN： 1996-0808

年卷期： 2011 年 5 卷 18 期

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收录情况： SCI

摘要： The polysaccharide fraction, CM-hs-CPS2, was isolated from fruiting bodies of cultured Cordyceps militaris grown on solid rice medium by hot water extraction and ethanol precipitation, and purified by ion exchange column (DEAE-cellulose-52) and gel filtration column (Sephadex G-100) chromatography. Its structural characterizations were analyzed by gas chromatograph (GC) and fourier transform infrared spectroscopy (FT-IR). Results indicated that CM-hs-CPS2 was a kind of sulfating acidic polysaccharide containing acetamido group. The monosaccharide composition analysis showed that CM-hs-CPS2 was composed mainly of mannose, glucose and galactose in a molar ration of 1.35: 8.34: 1.00, and linked by a-glycosidic linkage. The studies on antioxidant activities of CM-hs-CPS2 were evaluated by various methods in vitro. Results showed that DPPH. scavenging activity, reducing power and ferrous ion chelating activity of CM-hs-CPS2 (8 mg/ml) were 89%, 1.188 and 85%, respectively.

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