数字农科院2.0

Identification of target cells for Goose parvovirus infection in the immune system organs

文献类型： 外文期刊

作者： Zhu, L. Q.;Tao, J.;Wang, J. Y.;Zhang, X. J.;Wang, X. B.;Zhu, G. Q.;Ding, X. Y.;Ding, X. Y.

作者机构：

关键词： Derzsy's disease;Goose parvovirus;immunoassay;PCR

期刊名称： ACTA VIROLOGICA

ISSN： 0001-723X

年卷期： 2010 年 54 卷 3 期

页码：

收录情况： SCI

摘要： Target cells for Goose parvovirus (GPV) in natural infection are still unknown. In this study, immune system organs namely the spleen, bone marrow, thymus, bursa of Fabricius, and blood of experimentally GPV-infected goslings were examined by an immunoassay and flow cytometry for the presence of viral antigen and by a PCR for viral genome. The results indicated that the virus replicated in some cells of the spleen and bone marrow, but not in peripheral blood lymphocytes (PBLs). These data suggested that some cell populations in the spleen and bone marrow were targets for GPV infection. In addition, the immunoassay used for the detection of GPV was found comparable with a PCR in reliability and sensitivity.

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