数字农科院2.0

Sequence characterization, polymorphism, and chromosomal localizations of the porcine Capz genes

文献类型： 外文期刊

作者： Yang, E.;Wang, H.;Tang, Z. L.;Yang, S. L.;Li, K.;Yang, E.;Wang, H.;Wu, X. X.

作者机构：

关键词： porcine;physical mapping;polymorphism;association analysis;Capz

期刊名称： BIOCHEMICAL GENETICS

ISSN： 0006-2928

年卷期： 2008 年 46 卷 1-2 期

页码：

收录情况： SCI

摘要： CapZ is a widely distributed and highly conserved actin-binding protein that caps the barbed end of actin filaments and nucleates actin polymerization in a Ca2+-independent manner. In myofibrils, it is localized in the Z-lines. In this study, we cloned and characterized Capz subunit genes from the pig muscle. The nucleotide sequences and the predicted protein sequences share high sequence identity with other mammalian orthologs. The reverse transcriptase polymerase chain reaction (RT-PCR) revealed that porcine Capz beta, Capz alpha 1, and Capz alpha 2 genes are expressed in all 11 tissues studied (liver, spleen, small intestine, large intestine, lymph node, kidney, heart, skeletal muscle, brain, fat, and lung) but in variable amounts. Radiation hybrid mapping data indicated that Capz beta, Capz alpha 1, and Capz alpha 2 map to q2.1-2.6 of pig chromosome 6 (SSC6), q1.6-q2.2 of pig chromosome 4 (SSC4), and q1.3-2.3 of pig chromosome 18 (SSC18), respectively. An A/C single nucleotide polymorphism in Capz beta intron 4 was identified with a HhaI PCR restriction fragment length polymorphism, which showed great allele frequency differences between Guizhou Xiang, Guangxi Bama, Wuzhishan, Tongcheng, Landrace, and Yorkshire pigs. The association analysis suggested that the Capz beta genotype was associated with leaf fat (P < 0.05) in our experimental population.

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