数字农科院2.0

Cytogenetic analysis of F-1, F-2 and BC, plants from intergeneric sexual hybridization between Sinapis alba and Brassica oleracea by genomic in situ hybridization

文献类型： 外文期刊

作者： Wei, W. H.;Zhang, S. F.;Wang, L. J.;Li, J.;Chen, B.;Wang, Z.;Luo, L. X.;Fang, X. P.

作者机构：

关键词： Brassica oleracea;Sinapis alba;hybrid progenies;GISH

期刊名称： PLANT BREEDING

ISSN： 0179-9541

年卷期： 2007 年 126 卷 4 期

页码：

收录情况： SCI

摘要： By intergeneric sexual hybridization between Sinapis alba and Brassica oleracea, F-1, F-2 and BC1 progeny plants were produced. S. alba plants (genome SS, 2n = 24) were pollinated with B. oleracea (genome CC, 2n = 18), and the fertile F, plants were pollinated with B. oleracea to obtain BC, plants. GISH analysis showed that 10 out of 12 F, plants had 12 S. alba chromosomes (one full S chromosome set) and nine B. oleracea chromosomes (one C chromosome sets), representing the expected hybrids. However, two F-1 plants had 12 S chromosomes and 18 C chromosomes (two C chromosome sets), indicating unexpected hybrids. A maximum of three trivalents between C and S chromosomes were identified at metaphase I of semi-fertile F-1 pollen mother cells (PMCs), which indicates homology and chromosome pairing between these two genomes. The C genome had obviously been doubled in two F-2 plants from selfed semi-fertile F-1 plants. BC1 plants consisted of 18 C chromosomes and different numbers of one, five and six additional S chromosomes, respectively. Monosomic alien addition lines developed in the present study can be used for B. oleracea breeding and Sinapis alba gene mapping.

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