数字农科院2.0

IDENTIFICATION OF A SET OF RFLP PROBES FOR SUBSPECIES DIFFERENTIATION IN ORYZA-SATIVA L

文献类型： 外文期刊

作者： QIAN, HR;ZHUANG, JY;LIN, HX;LU, J;ZHENG, KL

作者机构：

关键词： RESTRICTION FRAGMENT LENGTH POLYMORPHISM (RFLP);PROBE SCREENING;SUBSPECIES DIFFERENTIATION;WIDE COMPATIBILITY VARIETY;ORYZA SATIVA L

期刊名称： THEORETICAL AND APPLIED GENETICS

ISSN： 0040-5752

年卷期： 1995 年 90 卷 6 期

页码：

收录情况： SCI

摘要： Sixty-eight indica-japonica tester-differentiating RFLP probes were tested in seven indica and seven japonica varieties of rice (Orysa sativa L.) with four enzyme digestions (EcoRI, EcoRV, HindIII and DraI). Twenty-one DNA clones were isolated as indica-japonica subspecies-differentiating probes. A set of 13 probes was established as core probes for subspecies differentiation and a pooled blotting analysis was carried out to facilitate the application of RFLP in rice genetics and breeding practice. A dendrogram of 12 wide-compatibility varieties was constructed based on RFLPs detected by 13 core probes with single enzyme digestions. It was speculated that most RFLPs of indica-japonica differentiating probes were generated by insertions/deletions, which may be of great significance for the origin and differentiation of subspecies in Oryza sativa L.

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