数字农科院2.0

Co-producing functional xylo-oligosaccharides and high-titer monosaccharides from Xanthoceras sorbifolia Bunge husks: A simple stepwise synergistic pretreatment

文献类型： 外文期刊

作者： Jingyi Xu;Yuxuan Yang;Kedong Ma;Minato Wakisaka;Zhiyong Ruan

作者机构：

关键词： Fed-batch enzymatic hydrolysis;Hydrothermal pretreatment;Lignocellulosic biomass;Mechanical milling;Xylo-oligosaccharides

期刊名称： Biomass and Bioenergy

ISSN： 0961-9534

年卷期： 2026 年 209 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Xanthoceras sorbifolium Bunge husk (XSH), a largely underutilized by-product of the woody oil industry, was converted into high-value xylo-oligosaccharides (XOS, DP 2–6) and fermentable monosaccharides via a novel integrated biorefinery strategy, which features simple operation, high efficiency, and strong industrial feasibility. Initially, rod milling (RM) combined with hydrothermal pretreatment (HP) was used as a chemical reagent-free and easy-to-operate process to solubilize XOS from XSH. This process achieved an XOS yield of 55.7 % with a low xylose/XOS ratio of 0.10, a result that not only ensures product purity but also reduces downstream processing costs. Subsequently, the pretreated solid residue was delignified with alkaline hydrogen peroxide (AHP) to improve cellulase accessibility, enabling batch enzymatic hydrolysis to produce glucose at a concentration of 104.1 g/L and a high yield of 94.6 %. Notably, the solid loading was set at 50 %, which meets industrial production requirements and is critical for reducing water consumption and separation costs. Under this condition, fed-batch enzymatic hydrolysis supplemented with Tween 80 yielded glucose and xylose at 290.6 g/L and 31.0 g/L, respectively, while overcoming common bottlenecks in high-solid processing. Mass balance analysis confirmed that 1000 g of XSH generated 115 g of XOS and 408 g of fermentable monosaccharides, verifying the feasibility of this value-added bioconversion route. This study demonstrates that XSH is a promising lignocellulosic feedstock, and the developed biorefinery strategy supports the large-scale production of high-value XOS and high-concentration fermentable sugars.

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