数字农科院2.0

Enhancement of immune responses to classical swine fever virus E2 in mice by fusion or mixture with the porcine IL-28B

文献类型： 外文期刊

作者： Hong Yuan;Yunjuan Jiao;Jie Gao;Tao Wang;Yingju Xia;Kun Li;Yuxuan Yang;Jing Zhang;Huifang Bao;Lihao Wang;Pu Sun;Dong Li;Pinghua Li;Yimei Cao;Zhixun Zhao;Zaixin Liu;Zengjun Lu;Yebing Liu;Xingwen Bai

作者机构：

关键词： Classical swine fever virus;E2 subunit vaccine;IL-28B

期刊名称： Applied microbiology and biotechnology

ISSN： 1432-0614

年卷期： 2025 年 109 卷 1 期

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： The E2 subunit vaccine has been considered a promising alternative to an attenuated classical swine fever (CSF) vaccine. However, it fails to induce a good cellular immune response. Given that immunogenic adjuvants can regulate the cellular immunity to achieve a maximum efficacy against antigens, immunostimulatory effects of porcine IL-28B on the CSF virus (CSFV) E2 subunit vaccine were evaluated in the present study. We expressed recombinant proteins E2-IL28B, E2, and IL-28B using CHO-S mammalian cells as an antigen expression platform, and three types of CSFV E2 subunit vaccines based on antigens E2-IL28B, E2 + IL-28B, and E2 were prepared, respectively. We found that both E2-IL28B and E2 + IL-28B antigens exhibited superior immunogenicity with dramatically induced antibody titers and neutralizing antibody levels than the E2 alone. Moreover, E2-IL28B or E2 + IL-28B, instead of E2, boosted cellular immune responses via obviously increasing the percentages of CD3+CD4+ T lymphocytes, promoting the lymphocyte proliferations, and enhancing the release of Th1-type cytokines. All results revealed that the inclusion of IL-28B, whether fused or mixed with E2, significantly elevated E2-induced immune potencies, suggesting that IL-28B could be used as a molecular adjuvant to optimize the design of E2 subunit vaccine for more effective controls of the CSF disease. KEY POINTS: • New CSF E2 subunit vaccine candidates were developed in which IL-28B was an immunoadjuvant • IL-28B significantly elevated the E2-induced immune potency whether it was fused or mixed with E2 • This study provided novel insights into the immunoregulatory properties of IL-28B used for the optimized subunit vaccine design.

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