数字农科院2.0

Bimetallic metal-organic frameworks with hydrolytic esterase activity applied to mycotoxin decontamination

文献类型： 外文期刊

作者： Ke Cheng;Peiheng Dina;Fei Yu;Yinghui Zhang;Yunpeng Wang;Wenjing Mu;Xiuguo Wang

作者机构：

关键词： Bimetallic artificial hydrolase;Broad substrate specificity;Esterase-like behavior;Patulin elimination

期刊名称： Chemical Engineering Journal

ISSN： 1385-8947

年卷期： 2026 年 530 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： Patulin (PAT), a hazardous mycotoxin, exhibits substantial health risks to humans and animals, encompassing mutagenic, teratogenic, and carcinogenic effects. It primarily contaminates fruit-derived products, thereby posing a severe threat to food safety, and demonstrates notable resistance to conventional decontamination methods. Here, we develop a bimetallic artificial hydrolase (Ni-MOF-808) by incorporating Ni2+ into the metal-organic framework MOF-808 via solvothermal synthesis. The integrated Ni2+ sites enhance the Lewis acidity of synthetic metalloenzymes, conferring superior hydrolysis activity toward a model substrate p-nitrophenyl acetate (p-NPA) containing ester group. Furthermore, Ni-MOF-808 demonstrates excellent environmental stability, maintaining high performance under extreme conditions, including prolonged storage, elevated temperatures, exposure to organic solvents, strongly acidic conditions and high ionic strength. Moreover, it exhibits outstanding reusability, retaining 95% of its initial catalytic activity after ten consecutive cycles. Importantly, Ni-MOF-808 displays low cytotoxicity and broad substrate specificity, with particular efficacy for the degradation of highly virulent PAT. Finally, the bimetallic nanozymes, rationally engineered into a three-layer structured continuous-flow reactor, exhibit admirable PAT elimination performance in complex juice matrices. Our methodology opens a pathway toward accurately designing esterase-like artificial enzymes with outstanding catalytic performance based on bimetallic active sites, and provides a promising strategy for addressing ester-containing mycotoxin contamination.

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