数字农科院2.0

Hyocholic Acid Species as the Key Modulator for Cecal Epithelial Homeostasis in Low-Birth-Weight Piglets

文献类型： 外文期刊

作者： Chang Yin;Xuan Liu;Wei Fang;Qingshi Meng;Xiaohui Feng;Weidong Zhang;Guoqi Dang;Ruqing Zhong;Liang Chen;Zirong Wang;Hongfu Zhang

作者机构：

关键词： bile acid;gut barrier function;hyocholic acid species;low birth weight

期刊名称： Nutrients

ISSN： 2072-6643

年卷期： 2025 年 17 卷 21 期

页码：

收录情况： SCIE（2025版）

摘要： Background: Low birth weight (LBW) is correlated with gut microbiota dysbiosis and intestinal barrier function disruption, increasing susceptibility to enteric diseases. These alterations underscore the critical need to identify key regulators of gut homeostasis, among which bile acids are increasingly recognized as pivotal for barrier integrity, microbial ecology, and host metabolism. Methods: Eight pairs of LBW (the initial BW was 0.850 ± 0.053 kg) and normal-birth-weight (NBW; 1.488 ± 0.083 kg) piglets were compared to evaluate cecal morphology and bile acid profiles. Subsequently, sixteen LBW piglets and eight NBW piglets were allocated into three groups: NBW (1.563 ± 0.052 kg), LBW control (LBW-CON; 0.950 ± 0.120 kg), and LBW with bile acid supplementation (LBW-bile powder; 0.925 ± 0.116 kg). Piglets in the LBW-bile powder group received 25 mg/kg BW of bile powder (hyodeoxycholic acid-enriched) by daily oral gavage for 14 days. Results: LBW piglets exhibited retarded cecal development and lower abundance of hyocholic acid species (p = 0.006). Importantly, bile powder supplementation significantly improved cecal length (p = 0.009) and mucosal thickness (p = 0.020) compared with LBW-CON piglets. Microbial analysis showed that the microbial dysbiosis index was restored to near-normal levels. Transcriptomic analysis revealed impaired extracellular matrix structure and mucus secretion in LBW piglets. Notably, bile powder supplementation markedly upregulated the protein expression of WNT8B (p < 0.001) and the bile acid receptors (i.e., GPBAR1 and FXR), alongside enhanced tight junctions and the goblet cell marker mucin-2 expression (p < 0.05). Conclusions: These findings suggest that specific bile acid supplementation improves gut barrier function and partially supports cecal development in LBW piglets.

分类号：

  • 相关文献

[1]Gut microbiota-derived ursodeoxycholic acid alleviates low birth weight-induced colonic inflammation by enhancing M2 macrophage polarization. Pi Y.,Wu Y.,Zhang X.,Lu D.,Han D.,Zhao J.,Zheng X.,Zhang S.,Ye H.,Lian S.,Bai Y.,Wang Z.,Tao S.,Ni D.,Zou X.,Jia W.,Zhang G.,Li D.,Wang J.. 2023

[2]Dietary ferulic acid and vanillic acid on inflammation, gut barrier function and growth performance in lipopolysaccharide-challenged piglets. Ruizhi Hu,Shusong Wu,Baizhen Li,Jijun Tan,Jiahao Yan,Ying Wang,Zhiyi Tang,Ming Liu,Chenxing Fu,Hongfu Zhang,Jianhua He. 2022

[3]Editorial: Dietary Polyphenols for Improving Gut Health: Volume 1. Kai Wang,Guiju Sun,Michael A. Conlon,Wenkai Ren,Guan Yang. 2021

[4]Mucin alleviates colonic barrier dysfunction by promoting spermine accumulation through enhanced arginine metabolism in Limosilactobacillus mucosae. Zhou, Xingjian,Xu, Qian,Zhang, Xiangyu,Wang, Hao,Bai, Yu,Wu, Yujun,Liu, Xiaoyi,Wang, Zhenyu,Hu, Jie,Huang, Mingyi,Pi, Yu,Zhao, Jinbiao,Wang, Junjun,Han, Dandan. 2024

[5]A Novel Synbiotic Protects Against DSS-Induced Colitis in Mice via Anti-inflammatory and Microbiota-Balancing Properties. Yang, Yong,Qiao, Yuyu,Liu, Ge,Chen, Weihao,Zhang, Ting,Liu, Jing,Fan, Weiping,Tong, Mingwei. 2024

[6]Effects Of Dietary Pectin On T.he Profile And Transport O f Intestinal Bile Acids In Young Pigs. Fang, W, Zhang, L, Meng, QS, Wu, WD, Lee, YK, Xie, JJ, Zhang, HF. 2018

[7]The Effect of Duck Bile Acid Extract on the Fecal Microbiota, Short Chain Fatty Acids, Fat Digestibility and Blood Biochemical Indices in Cats. Bing Han,Peng Zhao,Shukun Liang,Zhenlong Wang,Hui Tao,Xiumin Wang,Jie Liu,Jinquan Wang. 2023

[8]Exposure to High Aerial Ammonia Causes Hindgut Dysbiotic Microbiota and Alterations of Microbiota-Derived Metabolites in Growing Pigs. Shanlong Tang,Ruqing Zhong,Chang Yin,Dan Su,Jingjing Xie,Liang Chen,Lei Liu,Hongfu Zhang. 2021

[9]Impact Of Clinical Use Of P.arenteral Lipid Emulsions On B ile Acid Metabolism And Composition In Neonatal Piglets. Lavallee, CM, Lim, DW, Wizzard, PR, Mazurak, VC, Mi, S, Curtis, JM, Willing, BP, Yap, JY, Wales, PW, Turner, JM. 2019

[10]Supplementation of exogenous bile acids improve antitrichomonal activity and enhance intestinal health in pigeon (Columba livia). Hui Ma,Shixiong Bian,Pengmin Han,Yunlei Li,Aixin Ni,Ran Zhang,Pingzhuang Ge,Yuanmei Wang,Jinmeng Zhao,Yunhe Zong,Jingwei Yuan,Yanyan Sun,Jilan Chen. 2023

[11]Modulation of pectin on intestinal barrier function via changes in microbial functional potential and bile acid metabolism. Yin C.,Wen X.,Dang G.,Zhong R.,Meng Q.,Feng X.,Liu L.,Wu S.,He J.,Chen L.,Zhang H.. 2024

[12]Hydroxytyrosol Alleviates Intestinal Oxidative Stress by Regulating Bile Acid Metabolism in a Piglet Model. Xiaobin Wen,Fan Wan,Ruqing Zhong,Liang Chen,Hongfu Zhang. 2024

[13]Screening for potential warning biomarkers in cows with ketosis based on host–microbiota co-metabolism analysis. Zhenlong Du,Zhengzhong Luo,Yixin Huang,Tao Zhou,Li Ma,Dan Wu,Xueping Yao,Liuhong Shen,Shumin Yu,Kang Yong,Zuoting Yan,Suizhong Cao. 2024

[14]Decoding the role of the intestinal epithelium in hepatitis E virus infection using a human organoid prototype of “gut-liver” axis. Kuan Liu,Yang Wang,Jiangrong Zhou,Joy Joàn van der Meij,Luc J.W. van der Laan,Pengfei Li,Qiuwei Pan. 2025

[15]Lard reduces obesity in mice compared with corn oil and canola oil via modulating gut microbiota and bile acid metabolism. Li, Wusun,Wu, Weida,Zhang, Yuhui,Tang, Xiaoyan,Zheng, Xin,Huang, Xinyuan. 2025

[16]Dihydroquercetin attenuated Prevotella copri-caused intestinal injury by modulating gut microbiota and bile acids in weaned piglets. Long Wang,Ruizhi Hu,Siqi Ma,Xizi Yang,Jiatai Gong,Hongkun Xiang,Mingkun Shi,Xupeng Yuan,Liang Chen,Hongfu Zhang,Bie Tan,Xi He,Jianhua He,Shusong Wu. 2025

[17]Bile acid synthesis dysregulation in liver diseases promotes ectopic expansion of oral streptococci in the intestine. Yugui Wang,Wenjie Mu,Jian Guan,Pingping Ma,Yaqi Li,Ying Zhang,Wenjun Zhu,Yu Zhou,Yang Zou,Tongxu Zeng,Jian Zhou,Xiaoqi Lin,Xuehua Yan,Wenjuan Shi,Xiaola Guo,Xing Quan Zhu,Xuepeng Cai,Yan Sun,Aijiang Guo,Shuai Wang. 2025

作者其他论文 更多>>