数字农科院2.0

Polyphenols from Flos Trollii inhibit α-amylase and α-glucosidase: Kinetic analysis and mechanistic insights

文献类型： 外文期刊

作者： Xiaoai Zhu;Kebing Yan;Xianqing Ye;Xia Xiang;Yuan Ru;Yiying Niu;Yage Liu;Jun Xi;Kunlun Liu

作者机构：

关键词： Flos Trollii;Inhibition mechanism;Polyphenols;α-Amylase;α-Glucosidase

期刊名称： LWT

ISSN： 0023-6438

年卷期： 2025 年 225 卷

页码：

收录情况： SCIE（2025版） ; ; EI（2025版）

摘要： The increasing global prevalence of type 2 diabetes mellitus (T2DM) necessitates natural agents that target α-amylase and α-glucosidase to modulate starch digestion. Although Flos Trollii is a well-documented phytomedicine, its enzymatic inhibition profile remains understudied, particularly with respect to starch-digesting enzymes. This study employed an integrated approach combining UPLC−Q−TOF−MSE, enzyme kinetic, multispectral, and molecular docking simulations to elucidate the inhibitory mechanism of Flos Trollii polyphenols (FTP) on starch-digesting enzymes. UPLC−Q−TOF−MSE identified 10 major polyphenolic compounds. Kinetic analyses indicated that FTP inhibited α-amylase in mixed mode (IC50 = 67.70 ± 9.67 μg mL−1) and α-glucosidase in noncompetitive mode (IC50 = 36.13 ± 0.23 μg mL−1). FTP induced dynamic fluorescence quenching, resulting in the formation of enzyme-inhibitor complexes stabilized through hydrogen bonding, van der Waals interactions, and hydrophobic effects. Spectroscopic evidence confirmed that FTP binding triggered shifts in the enzyme structure, ultimately hampering its activity, whereas molecular docking further identified the specific binding sites. This study highlighted the potential of FTP as a nutraceutical for T2DM management.

分类号：

  • 相关文献

[1]Inhibition of α-amylase activity by quercetin via multi-spectroscopic and molecular docking approaches. Huang M.,Xiao Q.,Li Y.,Ahmad M.,Tang J.,Liao Q.,Tan C.. 2024

[2]Physicochemical, rheological properties and in vitro hypoglycemic activities of polysaccharide fractions from peach gum. Jiaxin Chen,Mo Zhou,Meng Liu,Jinfeng Bi. 2022

[3]Determination of potential α-glucosidase inhibitors in Jinxuan black tea based on bioaffinity ultrafiltration combined with UHPLC-Q-Exactive-MS. Guangneng Li,Jianyong Zhang,Hongchun Cui,Ying Gao,Yuliang Deng,Debao Niu,Junfeng Yin. 2025

[4]Rhizosphere Mortierella strain of alfalfa exerted weed growth inhibition by inducing expression of plant hormone-related genes. Du, Taotao,Qu, Xudong,Wang, Yibo,Li, Meixuan,Qie, Xihu,Jin, Jing,Gao, Yuxuan,Wang, Zengyu,Lin, Kejian,Yang, Chao,Sun, Juan. 2024

[5]Inhibitory effects of Lactobacillus brevis on Aspergillus westerdijkiae and antifungal compounds identification. Li Li,Bolei Yang,Shuo Yang,Xiaoyu Tian,Yuan Gao,Jun Song,Wang Haifeng,Tiejun Li,Fuguo Xing. 2024

[6]Nontargeted and targeted metabolomics analysis for evaluating the effect of “golden flora” amount on the sensory quality, metabolites, and the alpha-amylase and lipase inhibitory activities of Fu brick tea. Yingying Li,He Zhou,Tian Tian,Yihong Hou,Dan Chen,Jie Zhou,Shuyuan Liu,Youben Yu,Weidong Dai,Tianshan Zhou. 2023

[7]Analysis of the mechanism of resistance to enzymatic hydrolysis of RS-5 resistant starch. Haixia Zhong,Yongxin She,Xijuan Yang,Qiao Wen,Li Chen,Xue Bo Wang,Zhiguang Chen. 2024

[8]Identification and characterization of a calcium-binding peptide from salmon bone for the targeted inhibition of α-amylase in digestion. Zhe Xu,Shiying Han,Na Cui,Hanxiong Liu,Xu Yan,Hongrui Chen,Jianping Wu,Zhijian Tan,Ming Du,Tingting Li. 2024

[9]Analysis of the interaction between double-helix starch molecule and α-amylase. Zhong Haixia,She Yongxin,Yang Xijuan,Wen Qiao,Chen Li,Wang XueBo,Chen Zhiguang. 2024

[10]Genome-Wide Identification and Expression Profiling of the α-Amylase (AMY) Gene Family in Potato. Yudan Duan,Liping Jin. 2024

[11]Analysis of the relationship between starch molecular conformation and enzymatic hydrolysis efficiency. Zhong Haixia,Yang Xijuan,She Yongxin,Gan Guochao,Wen Qiao,Chen Li,Chen Zhiguang. 2024

[12]Suppression of Bta11975, an α-glucosidase, by RNA interference reduces transmission of tomato chlorosis virus by Bemisia tabaci. Ding Yi Hui Lu,Hao Yue,Li Ping Huang,De Yong Zhang,Zhan Hong Zhang,Zhuo Zhang,Youjun Zhang,Fan Li,Fei Yan,Xu Guo Zhou,Xiao Bin Shi,Yong Liu. 2021

[13]Chemical profile of phenolic extracts from rapeseed meal and inhibitory effects on α-glucosidase: UPLC-MS/MS analysis, multispectral approaches, molecular simulation and ADMET analysis. Huihui Liu,Ziliang Li,Xiaoyang Xia,Ruiying Zhang,Wen Wang,Xia Xiang. 2023

[14]Exploring formation of turanose in honey via stable isotope labelling and high-resolution mass spectrometry analysis. Yuzhe Yuan,Sha Yan,Liming Wu,Haitao Xu,Guodong Mu,Xiaofeng Xue. 2024

[15]Surface engineering of carbon dots for highly sensitive α-glucosidase assay and inhibition evaluation. Meijuan Liang,Gege Song,Yeqing Wan,Yingying Chen,Fuan Wang,Xiaoqing Liu. 2024

[16]Extraction effects of eight deep eutectic solvents on dianhong black tea: From chemical composition analysis to antioxidant and α-glucosidase inhibitory assessments. Zixin Zhao,Mingchun Wen,Zongde Jiang,Shengxiao Su,Yong Quan Xu,Zisheng Han,Yong Cheng,Mengting Zhu,Liang Zhang. 2024

[17]Polyphenols in whole rice grain: Genetic diversity and health benefits. Shao, Yafang,Shao, Yafang,Bao, Jinsong.

[18]Micronutrients-fortified rapeseed oil improves hepatic lipid accumulation and oxidative stress in rats fed a high-fat diet. Xu, Jiqu,Deng, Qianchun,Huang, Qingde,Yang, Jin'e,Huang, Fenghong,Xu, Jiqu,Deng, Qianchun,Huang, Qingde,Yang, Jin'e,Huang, Fenghong,Zhou, Xiaoqi,Gao, Hui,Chen, Chang,Ma, Jing,Wan, Zhengyang. 2013

[19]Characterization of Polyphenols from Lycium ruthenicum Fruit by UPLC-Q-TOF/MSE and Their Antioxidant Activity in Caco-2 Cells. Wu, Tao,Lv, Haiyang,Wang, Fengzhong,Wang, Yi.

[20]NtPHYB1(K326), a homologous gene of Arabidopsis PHYB, positively regulates the content of phenolic compounds in tobacco. Zhao, Jiehong,Zhang, Jie,Li, Zhenhua,Yu, Jing,Yu, Shizhou,Guo, Yushuang,Fu, Yongfu,Zhang, Xiaomei,Han, Jie.

作者其他论文 更多>>