数字农科院2.0

Molecular Characterization And Genomic Function Of Grapevine Geminivirus A

文献类型： 外文期刊

作者： Sun, SW; Hu, Y; Jiang, GZ; Tian, YM; Ding, M; Yu, C; Zhou, XP; Qian, YJ

作者机构：

关键词： Grapevine Geminivirus A; Defective Subviral Molecule; Pathogenicity; Genetic Variability; Genetic Evolution

期刊名称： FRONTIERS IN MICROBIOLOGY

ISSN： 1664-302X

年卷期： 2020 年 11 卷

页码：

收录情况： JCR（2021版）

摘要： A new grapevine geminivirus A (GGVA) isolate (named as GGVA-17YM1) and its associated defective genome (GGVA-D) were identified from a grapevine sample collected in Yuanmou, Yunnan Province, using sRNA high throughput sequencing and traditional Sanger sequencing. To explore the pathogenicity of GGVA and GGVA-D, infectious clones of GGVA-17YM1 and GGVA-D-17YM1 were constructed. Infection assays indicated thatNicotiana benthamianaplants inoculated with GGVA alone or a combination of GGVA and GGVA-D exhibited upward curled apical leaves and dwarfism. Southern blotting and quantitative real-time polymerase chain reaction analysis revealed that GGVA-D increased the accumulation level of GGVA DNA. Transient expression using a PVX-derived recombinant vector indicated that C2 and C4 encoded by GGVA are involved in symptom induction inN. benthamiana. Furthermore, the V2 protein inhibited local RNA silencing in co-infiltration assays inGFPtransgenicN. benthamianaplants. Subsequently, full-length genome sequencing resulted in the identification of 11 different isolates of GGVA and 9 associated defective DNA molecules. Phylogenetic analysis based on whole genome sequences showed that all GGVA isolates, including our sequences, clustered into two distinct branches with no geographical grouping. Analyses of molecular variation indicated single nucleotide polymorphisms (SNPs) with more transitions (55.97%) than transversions (44.03%). Furthermore, the main variants for ORF C1, C3, or V1 were synonymous mutations, and non-synonymous mutations for ORF C2, C4, and V2. Genetic selection analysis indicated that negative selection acted on four ORFs (V1, C1, C2, and C3), while V2 and C4 were under positive selection. Our results contribute to the characterization of the genetic diversity of GGVA and provide insights into its pathogenicity.

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