数字农科院2.0

Gma-miR398c/d negatively regulates soybean resistance to Soybean mosaic virus by targeting SOD family genes

文献类型： 外文期刊

作者： Li, Bowen;Wang, Liqun;Qian, Xueyan;Liu, Hui;Jin, Tongtong;Yin, Jinlong;Hu, Ting;Liu, Mengzhuo;Guo, Dongquan;Li, Kai;Gai, Junyi;Zhi, Haijian

作者机构：

关键词： Soybean;Soybean mosaic virus;MiR398;Target gene;Reactive oxygen species

期刊名称： CROP JOURNAL

ISSN： 2095-5421

年卷期： 2025 年 13 卷 5 期

页码：

收录情况： SCIE（2025版） ; ; CSCD（2025-2026年度） ; ; 科技核心（2024版） ; ; 农林核心（2024版）

摘要： Soybean mosaic virus (SMV) poses a substantial threat to the yield and quality of soybean (Glycine max (L.) Merr.), leading to significant economic losses in soybean production. However, the mining of SMVresistance loci and the exploration of the underlying disease resistance mechanisms remain relatively limited. MicroRNAs (miRNAs) are a class of post-transcriptional regulators that play a pivotal role in modulating plant growth, development and responding to various stresses. In this study, we demonstrated the function of the miR398c/d-GmCSDs module between soybean resistant and susceptible varieties, focusing on its differential regulatory roles in SMV infection. Specifically, SMV infection downregulated gma-miR398c/d expression in the resistant variety (Qihuang 1, QH), while upregulated them in the susceptible variety (Nannong 1138-2, NN). Transient expression assay in N. benthamiana confirmed that gma-miR398c/d can target six superoxide dismutase (SOD) family genes, which responded to SMV infection in both varieties. Stable overexpression of Gma-MIR398c/d in soybean or inhibition of the corresponding target genes' expression via Bean pod mottle virus (BPMV)-induced gene silencing (VIGS) led to reduced H2O2 content and thereby promoted SMV infection. Conversely, plants overexpressing the target genes exhibited the opposite phenotypes. The functions of gma-miR398c/d and their target genes were further validated in N. benthamiana through transient co-expression with SMV infectious clone (pSC7-GFP), indicating that gma-miR398c/d negatively regulated soybean resistance to SMV, while the target genes positively contributed to disease resistance. Collectively, our findings provide novel insights into the regulatory mechanisms underlying soybean resistance to SMV. (c) 2025 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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