数字农科院2.0

Bioelectrochemical Fixation of Nitrogen to Extracellular Ammonium by Pseudomonas stutzeri

文献类型： 外文期刊

作者： Shanshan Chen;Xianyue Jing;燕永亮;Shaofu Huang;Xing Liu;Piao Chen;Shungui Zhou

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关键词： bioelectrochemical system; electron uptake mechanism; nitrogen fixation; phenazine; Pseudomonas stutzeri

期刊名称： Appl. Environ. Microbiol.

ISSN： 0099-2240

年卷期： 2021 年 87 卷 5 期

页码：

收录情况： JCR（2021版） ; EI（2021版）

摘要： Diazotrophs can produce bioavailable nitrogen from inert N2 gas by bioelectrochemical nitrogen fixation (e-BNF), which is emerging as an energy-saving and highly selective strategy for agriculture and industry. However, current e-BNF technology is impeded by requirements for NH4+ assimilation inhibitors to facilitate intracellular ammonia secretion and precious metal catalysts to generate H2 as the energycarrying intermediate. Here, we initially demonstrate inhibitor- and catalystless extracellular NH4+ production by the diazotroph Pseudomonas stutzeri A1501 using an electrode as the sole electron donor. Multiple lines of evidence revealed that P. stutzeri produced 2.3260.25 mg/liter extracellular NH4+ at a poised potential of 20.3 V (versus standard hydrogen electrode [SHE]) without the addition of inhibitors or expensive catalysts. The electron uptake mechanism was attributed to the endogenous electron shuttle phenazine-1-carboxylic acid, which was excreted by P. stutzeri and mediated electron transfer from electrodes into cells to directly drive N2 fixation. The faradaic efficiency was 20% 6 3%, which was 2 to 4 times that of previous e-BNF attempts using the H2-mediated pathway. This study reports a diazotroph capable of producing secretable NH4+ via extracellular electron uptake, which has important implications for optimizing the performance of e-BNF systems and exploring the novel nitrogen-fixing mode of syntrophic microbial communities in the natural environment. © 2021 American Society for Microbiology. All Rights Reserved.

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