数字农科院2.0

Functional Characterization of Dual-Initiation Codon-Derived V2 Proteins in Tomato Yellow Leaf Curl Virus

文献类型： 外文期刊

作者： Zhiyuan Wang;Pan Gong;Siwen Zhao;Fangfang Li;Xueping Zhou

作者机构：

关键词： gene silencing suppression;host-pathogen interaction;tomato yellow leaf curl virus (TYLCV);V2 protein isoforms;viral pathogenesis

期刊名称： Agronomy

ISSN： 2073-4395

年卷期： 2025 年 15 卷 7 期

页码：

收录情况： SCIE（2025版）

摘要： Tomato yellow leaf curl virus (TYLCV) is a highly destructive pathogen of global tomato crops. The open reading frame (ORF) of TYLCV V2 contains two initiation codons (ATG1/V2-1 and ATG2/V2-2), producing distinct protein isoforms. Using custom antibodies, we confirmed V2-1 and V2-2 expression in infected Nicotiana benthamiana and tomato plants. Deletion mutants revealed their specialized roles: V2-1 was indispensable for viral replication and systemic spread—its loss severely reduced pathogenicity and genome accumulation. V2-2 acted as an auxiliary factor, and its deletion attenuated symptoms but kept the virus infection. Host-specific effects were observed—V2-1 deletion led to lower viral DNA/coat protein levels in N. benthamiana than in tomato, suggesting host-dependent regulation. Mutant viruses declined progressively in tomato, indicating host defense clearance. Heterologous co-expression of both isoforms via potato virus X induced systemic necrosis in N. benthamiana, demonstrating functional synergy between isoforms. Both initiation codons were essential for V2-mediated suppression of transcriptional gene silencing (TGS) and post-transcriptional gene silencing (PTGS). This study uncovers the mechanistic divergence of V2 isoforms in TYLCV infection, highlighting their collaborative roles in virulence and host manipulation. The findings advance understanding of geminivirus coding complexity and offer potential targets for resistance strategies.

分类号：

  • 相关文献

[1]Insecticides promote viral outbreaks by altering herbivore competition. Pan, Huipeng,Wang, Shaoli,Wu, Qingjun,Zhang, Youjun,Preisser, Evan L.,Chu, Dong,Carriere, Yves,Pan, Huipeng,Zhou, Xuguo.

[2]The coat protein of the ilarvirus prunus necrotic ringspot virus mediates long-distance movement. Chengyong He,Fei Xing,Xiaoli Zhao,Shifang Li,Binhui Zhan,Zhen Liu,Tengfei Xu,Dehang Gao,Zhenfei Dong,Hongqing Wang,Zhixiang Zhang. 2023

[3]Newcastle disease virus exploits the phospholipid flippase ATP11c–CDC50A complex to promote viral infection. Dandan Zhang,Yuechi Hou,Xusheng Qiu,Yang Qu,Yingjie Sun,Cuiping Song,Ying Liao,Chan Ding,Lei Tan. 2025

[4]Single-Cell Atlas of Spleen Remodeling Reveals Macrophage Subset-Driven ASFV Pathogenesis. Liyuan Wang,Shouzhang Sun,Lei Liu,Yun Chen,Haixue Zheng,Zhonglin Tang. 2025

[5]The glycosylation variant at residue 381 of the spike protein contributes to virulence shifts in porcine epidemic diarrhea virus during both natural field transmission and laboratory cell passaging with poor cross-protection. Li, Zhiwei,Ma, Zhiqian,Li, Yongqi,Zhao, Xiaojing,Zheng, Yonghui,Li, Yang,Feng, Yingtong,Guo, Xuyang,Zheng, Zifang,Xu, Lele,Zhang, Jianwu,Zheng, Haixue,Xiao, Shuqi. 2025

[6]Toxoplasma gondii infection and schizophrenia: an inter-kingdom communication perspective. Elsheikha, Hany M.,Zhu, Xing-Quan.

[7]Dual Identification and Analysis of Differentially Expressed Transcripts of Porcine PK-15 Cells and Toxoplasma gondii during in vitro Infection. Zhou, Chun-Xue,Suo, Xun,Zhou, Chun-Xue,Suo, Xun,Zhou, Chun-Xue,Zhou, Dong-Hui,Liu, Qing,Zhu, Xing-Quan,Elsheikha, Hany M.,Zhu, Xing-Quan. 2016

[8]De novo transcriptome sequencing and analysis of the juvenile and adult stages of Fasciola gigantica. Zhang, Xiao-Xuan,Ma, Jian-Gang,Zhao, Quan,Zhang, Xiao-Xuan,Cong, Wei,Ma, Jian-Gang,Zhu, Xing-Quan,Cong, Wei,Elsheikha, Hany M.,Liu, Guo-Hua,Huang, Wei-Yi,Zhu, Xing-Quan.

[9]The known and missing links between Toxoplasma gondii and schizophrenia. Elsheikha, Hany M.,Busselberg, Dietrich,Zhu, Xing-Quan. 2016

[10]A Novel Pepper (Capsicum annuum L.) WRKY Gene, CaWRKY30, Is Involved in Pathogen Stress Responses. Zheng Jingyuan,Mao Zhenchuan,Xie Bingyan,Zheng Jingyuan,Zou Xuexiao.

[11]Toxoplasma gondii induces metabolic disturbances in the hippocampus of BALB/c mice. Jun Ma,Jun Jun He,Meng Wang,Jun Ling Hou,Hany M. Elsheikha,Xing Quan Zhu. 2021

[12]Functional Characterization of 17 Protein Serine/Threonine Phosphatases in Toxoplasma gondii Using CRISPR-Cas9 System. Qin Li Liang,Lan Bi Nie,Ting Ting Li,Hany M. Elsheikha,Li Xiu Sun,Zhi Wei Zhang,Dan Yu Zhao,Xing Quan Zhu,Jin Lei Wang. 2022

[13]Suppression of plant immunity by Verticillium dahliae effector Vd6317 through AtNAC53 association. Liu, Lisen,Li, Jianing,Wang, Zhaohan,Zhou, Haodan,Wang, Ye,Qin, Wenqiang,Duan, Hongying,Zhao, Hang,Ge, Xiaoyang. 2024

[14]Reassessing Schistosoma worms: the overlooked role in host pathology and disease elimination. Haoran Zhong,Zhiqiang Fu,Jinming Liu,Yamei Jin. 2025

[15]Verticillium dahliae effector Vd06254 disrupts cotton defence response by interfering with GhMYC3-GhCCD8-mediated hormonal crosstalk between jasmonic acid and strigolactones. Ma, Jianhui,Jiang, Fan,Yu, Yan,Zhou, Haodan,Zhan, Jingjing,Li, Jianing,Chen, Yanli,Wang, Ye,Duan, Hongying,Ge, Xiaoyang,Xu, Zhenzhen,Zhao, Hang,Liu, Lisen. 2025

[16]In the coevolution of cotton and pathogenic fungi, resistant cotton varieties lead to an escalation in the virulence of Verticillium dahliae. Yalin Zhang,Lifang Cui,Ruibing Liu,Zili Feng,Hongjie Feng,Jinglong Zhou,Lihong Zhao,Feng Wei,Heqin Zhu. 2025

[17]Toxoplasma gondii PPM3H regulates the parasite virulence and modulates host immune and inflammatory responses in mice. He, Jun-Jun,Ma, Jun,Deng, Meng-Ling,Elsheikha, Hany M.,Wang, Yi-Dan,Zhang, Yu-Cong,Zou, Feng-Cai,Zhu, Xing-Quan. 2025

[18]Quorum-sensing regulator LsrR modulates avian pathogenic Escherichia coli pathogenicity through direct regulation of cysN. Nawaz, Saqib,Wang, Zhihao,Jiang, Wei,Kong, Lanfang,Yin, Huifang,Bao, Yinli,Huang, Cuiqin,Chen, Zhaoguo,Zhang, Yan,Han, Xiangan. 2025

作者其他论文 更多>>