数字农科院2.0

Pseudomonas cold shock proteins suppress bacterial effector translocation in Nicotiana benthamiana

文献类型： 外文期刊

作者： Shen Cong;Jun Zhou Li;Mei Ran Zhang;Hai Lei Wei;Wei Zhang

作者机构：

关键词： bacterial effector translocation;cold shock proteins;Nicotiana benthamiana;plant immunity;Pseudomonas

期刊名称： Frontiers in Microbiology

ISSN： 1664-302X

年卷期： 2025 年 16 卷

页码：

收录情况： SCIE（2025版）

摘要： Introduction: Plants detect the invasion of microbial pathogens through pathogen-associated molecular patterns (PAMPs). Cold shock proteins (CSPs) are a class of PAMPs specifically recognized by Solanales plants. While peptide inoculation studies have revealed the effects of CSPs, their in vivo roles remain poorly understood. Methods: A model system involving the interactions between Pseudomonas fluorescens and P. syringae pv. tomato DC3000 with Nicotiana benthamiana has been widely used to investigate the molecular mechanism of plant-microbe interactions. Here, we employed this model system to explore the in vivo roles of CSPs in modulating plant immunity by multiple genetic approaches. Results: Our findings revealed that three highly-conserved CSPs were identified in Pseudomonas strains. Transient expression of these CSPs neither induced reactive oxygen species (ROS) production nor suppressed the hypersensitive response (HR) in N. benthamiana, however, it restricted bacterial effector translocation. Genetic analysis revealed that these CSPs did not contribute to the ROS burst or HR inhibition in vivo but were functionally redundant in suppressing effector translocation in a flagellin (FliC)-independent manner. Furthermore, we demonstrated that the suppression of effector translocation mediated by CSPs was less pronounced compared to that triggered by FliC. Additionally, inoculation with csp15 and csp22 epitopes triggered the pattern-triggered immunity-associated suppression of effector translocations. Discussion: This study revealed the redundant roles of CSPs in suppressing bacterial effector translocation in vivo, providing deep insights into the PTI elicited by cytoplasmic bacterial proteins.

分类号：

  • 相关文献

[1]Duplicated Flagellins in Pseudomonas Divergently Contribute to Motility and Plant Immune Elicitation. Luo, Yuan,Wang, Jing,Gu, Yi-Lin,Zhang, Li-Qun,Wei, Hai-Lei. 2023

[2]植物青枯菌（Pseudomonassolanacearum）致病机理. 康耀卫. 1995

[3]High-Level Accumulation of Exogenous Small RNAs Not Affecting Endogenous Small RNA Biogenesis and Function in Plants. Shen Wan-xia,Zhou Chang-yong,Shen Wan-xia,Smith, Neil A.,Wang Ming-bo,Shen Wan-xia,Zhou Chang-yong. 2014

[4]Identification of photoperiod-regulated gene in soybean and functional analysis in Nicotiana benthamiana. Sha Ai-Hua,Shan Zhi-Hui,Zhang Xiao-Juan,Wu Xue-Jun,Qiu De-Zheng,Zhou Xin-An,Chen Yin-Hua,Sha Ai-Hua,Shan Zhi-Hui,Zhang Xiao-Juan,Wu Xue-Jun,Qiu De-Zheng,Zhou Xin-An.

[5]A wheat (Triticum aestivum) protein phosphatase 2A catalytic subunit gene provides enhanced drought tolerance in tobacco. Xu, Chongyi,Jing, Ruilian,Mao, Xinguo,Jia, Xiaoyun,Chang, Xiaoping.

[6]Mimic Phosphorylation of a beta C1 Protein Encoded by TYLCCNB Impairs Its Functions as a Viral Suppressor of RNA Silencing and a Symptom Determinant. Zhong, Xueting,Wang, Zhan Qi,Xiao, Ruyuan,Cao, Linge,Wang, Yaqin,Xie, Yan,Zhou, Xueping,Zhou, Xueping.

[7]Plant ERD2-like proteins function as endoplasmic reticulum luminal protein receptors and participate in programmed cell death during innate immunity. Li, Sizhun,Xie, Ke,Zhang, Qiang,Wang, Yan,Tang, Yang,Liu, Dong,Liu, Yule,Hong, Yiguo,He, Chenyang. 2012

[8]Analysis of lysine acetylation in tomato spot wilt virus infection in Nicotiana benthamiana. Yanwei Gong,Ying Li,Dongyang Liu,Lianqiang Jiang,Hui Liang,Yuanhua Wu,Fenglong Wang,Jinguang Yang. 2023

[9]SAMDC3 enhances resistance to Barley stripe mosaic virus by promoting the ubiquitination and proteasomal degradation of viral gamma b protein. Li, Zhaolei,Yang, Xinxin,Li, Wenli,Wen, Zhiyan,Duan, Jiangning,Jiang, Zhihao,Zhang, Dingliang,Xie, Xialin,Wang, Xueting,Li, Fangfang,Li, Dawei,Zhang, Yongliang. 2022

[10]Genome-wide analysis of MYB family in Nicotiana benthamiana and the functional role of the key members in resistance to Bemisia tabaci. Feng Bin Zhang,Shun Xia Ji,Jin Guang Yang,Xiao Wei Wang,Wen Hao Han. 2023

[11]Identification And Analysis Of Potential G.enes Regulated By An A lphasatellite (Tylccna) That Contribute To Host Resistance Against Tomato Yellow Leaf Curl China Virus And Its Betasatellite (Tylccnv/Tylccnb) Infection In. Zhou, Xueping,Zhou, Xueping,Liu, Xianan,Zhao, Liling,Luo, Chaohu,Wang, Zhan Qi,Xie, Yan. 2019

[12]Effects of different light conditions on transient expression and biomass in Nicotiana benthamiana leaves. Yuejing Zhang,Yi Ru,Zhenzhen Shi,Hanqi Wang,Ji Zhang,Jianping Wu,Hailong Pang,Hanqing Feng. 2023

[13]Biochemical characterization of acyl-CoA:diacylglycerol acyltransferase2 from the diatom Phaeodactylum tricornutum and its potential effect on LC-PUFAs biosynthesis in planta. Sylwia Klińska-Bąchor,Kamil Demski,Yangmin Gong,Antoni Banaś. 2024

[14]A Colletotrichum tabacum Effector Cte1 Targets and Stabilizes NbCPR1 to Suppress Plant Immunity. Yuan Xue,Shouhui Pan,Quan Zhang,Fei Dai,Junxiang Zhang. 2024

[15]Unveiling protein elicitor MoHrip2: A potent biocontrol agent extracted from Magnaporthe oryzae safeguards Australian tobacco (Nicotiana benthamiana) against tobacco aphid (Myzus nicotianae). Khadija Javed,Humayun Javed,Qiu Dewen,Yong Wang. 2024

[16]The root-knot nematode effector MiEFF12 targets the host ER quality control system to suppress immune responses and allow parasitism. Salomé Soulé,Kaiwei Huang,Karine Mulet,Joffrey Mejias,Jérémie Bazin,Nhat My Truong,Junior Lusu Kika,Stéphanie Jaubert,Pierre Abad,Jianlong Zhao,Bruno Favery,Michaël Quentin. 2024

[17]Genome-wide analysis of the WRKY family in Nicotiana benthamiana reveals key members regulating lignin synthesis and Bemisia tabaci resistance. Shun Xia Ji,Feng Bin Zhang,Hong Da Song,Kai Lu Liang,Jun Xia Wang,Xin Yue Zhang,Xiao Wei Wang,Jin Guang Yang,Wen Hao Han. 2024

[18]Editing of an antiviral host factor boosts plant growth and yield of plant viral vector-mediated heterologous protein expression. Fang, Zhu,Zhao, Xinru,Du, Min,Xu, Xinyi,Zhou, Hui,Guo, Wei,Zhou, Xueping,Yang, Xiuling. 2025

[19]Multi-Omics Analyses Reveal the Red and Far-Red Light Combination Enhancing Heterologous Protein and Metabolite Production in Nicotiana benthamiana. Zhang, Yating,Wang, Fengjiao,Du, Ran,Li, Tao,Zhou, Shaoqun,Yan, Jianbin,Li, Wei. 2025

[20]Culture filtrates derived from endophytes Epicoccum spp. confer resistance against tobacco mosaic virus in Nicotiana benthamiana. Qi An,Clement Nzabanita,Lihang Zhang,Yanfei Wang,Shuangchao Wang,Lihua Guo,Yan Zhao. 2025

作者其他论文 更多>>