数字农科院2.0

Gold core @ platinum shell nanozyme-mediated magnetic relaxation switching DNA sensor for the detection of Listeria monocytogenes in chicken samples

文献类型： 外文期刊

作者： Zijing Wu;Chenxi Huang;Yongzhen Dong;Binjie Zhao;Yiping Chen

作者机构：

关键词： Au@Pt nanozymes;DNA hybridization;Hydrogen peroxide;Listeria monocytogenes;Magnetic relaxation switching;Mn (VII)/Mn (II) conversion

期刊名称： Food Control

ISSN： 0956-7135

年卷期： 2022 年 137 卷

页码：

收录情况： SCIE（2022版）

摘要： Herein, an Au@Pt nanozyme-mediated magnetic relaxation switching (MRS) DNA biosensor has been developed for the rapid detection of Listeria monocytogenes. Au@Pt nanoparticle was with enhanced stability and high peroxidase activity for decomposing the hydrogen peroxide. After DNA hybridization and magnetic separation, the conjugates of MNP180-probe1-L. monocytogenes DNA-probe2-Au@Pt could adjust the hydrogen peroxide-mediated Mn(VII)/Mn(II) conversion, and thus leading to the change of the transverse relaxation time. The amount of Au@Pt was directly proportional to the concentration of L. monocytogenes. The Au@Pt nanozyme and the hydrogen peroxide-mediated Mn(VII)/Mn(II) signal conversion system contributed to the sensitivity and stability of this sensor. This sensor enabled the detection of L. monocytogenes with a LOD of 30 CFU/mL, which was 33-folds enhancement compared with that of enzyme-linked immunosorbent assay, providing a promising platform to trace the foodborne pathogen in the complex food matrix.

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