数字农科院2.0

Multiplexed and DNA amplification-free detection of foodborne pathogens in egg samples: Combining electrical resistance-based microsphere counting and DNA hybridization reaction

文献类型： 外文期刊

作者： Xiaobo Hu;Xinrui Cheng;Zhilong Wang;Junpeng Zhao;Xufeng Wang;Weihai Yang;Yiping Chen

作者机构：

关键词： DNA hybridization Reaction;Foodborne pathogens;Microsphere counter;Multiplex detection;Oligonucleotide probe

期刊名称： Analytica Chimica Acta

ISSN： 0003-2670

年卷期： 2022 年 1228 卷

页码：

收录情况： SCIE（2022版） ; EI（2022版）

摘要： A multiplex and DNA amplification-free sensor based on electrical resistance microsphere counter (ERMC) and DNA hybridization reaction has been developed for simultaneous detection of Listeria monocytogenes, Salmonella Typhimurium, and Staphylococcus aureus in egg samples. In this strategy, magnetic nanoparticles (MNPs) and polystyrene microspheres (PMs) were labeled with DNA probes, the PMs with different sizes (3 μm, 4 μm and 6 μm) were used as multiplex signal reporters due to the high resolution of ERMC. After magnetic separation, the number of unbound PM-probe was related to the concentration of pathogens, and their sizes were attributed to types of pathogens. The limit of detection of this sensor was 20 CFU/mL for Listeria monocytogenes, 89 CFU/mL for Salmonella, and 94 CFU/mL for Staphylococcus aureus, respectively. The DNA amplification-free, simplicity and low-cost of this assay make it a promising tool for the rapid screening of multiple pathogens in food samples.

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