数字农科院2.0

Targeted deletion of three CYP51s in Fusarium fujikuroi and their different roles in determining sensitivity to 14a-demethylase inhibitor fungicides

文献类型： 外文期刊

作者： Mao, Cheng-Xin;Luo, Ju;Zhang, Yu;Zhang, Chuan-Qing

作者机构：

关键词： Fusarium fujikuroi;14 alpha-demethylase inhibitors;Fusarium graminearum;prochloraz;Cyp51

期刊名称： PEST MANAGEMENT SCIENCE

ISSN： 1526-498X

年卷期： 2023 年

页码：

收录情况： SCIE（2023版）

摘要： Background Fusarium fujikuroi is the pathogenic agent of rice bakanae disease and has developed serious resistance to prochloraz, a 14 alpha-demethylase inhibitor (DMI). Prochloraz resistance in F. fujikuroi is caused by cooperation between FfCyp51B with Cyp51A and shows cross-resistance only to prothioconazole but not to tebuconazole, difenoconazole, propiconazole, metconazole, hexaconazole, and triadimefon. This study aimed to analyze the functions of the three Cyp51s in F. fujikuroi, especially their role in determining sensitivity to DMIs.Results The respective deletion of FfCyp51A, Cyp51B, and Cyp51C had no obvious effect on morphology, conidium germination, or pathogenicity. The involvement of growth, growth and ergosterol biosynthesis, and conidium production and ergosterol biosynthesis was observed for FfCyp51A, Cyp51B, and Cyp51C, respectively. Compared with the sensitive isolate of F. fujikuroi, the effect on sensitivity to the tested DMIs was divided into four groups: (i) both of Cyp51A and Cyp51B positively regulate the sensitivity to prochloraz and prothioconazole; (ii) Cyp51B positively regulate the sensitivity to tebuconazole and metconazole, but negatively regulate the sensitivity to difenoconazole; (iii) Cyp51A and Cyp51B play opposite roles in the sensitivity to triadimefon. Therefore, deletion of Cyp51A in F. fujikuroi confers a higher sensitivity to triadimefon, while deletion of Cyp51B results in a triadimefon-resistant mutant isolate; (iv) deletion of Cyp51B yielded a mutant isolate that was more resistant to propiconazole and hexaconazole.Conclusion Sophisticated interactions exist within the three Cyp51 genes to DMIs fungicides sensitivity in F. fujikuroi, and Cyp51B probably plays a more critical role than Cyp51A and Cyp51C. (c) 2022 Society of Chemical Industry.

分类号：

  • 相关文献

[1]Size-Dependent Effect of Prochloraz-Loaded mPEG-PLGA Micro- and Nanoparticles. Zhang, Jiakun,Liu, Yajing,Cao, Lidong,Huang, Qiliang,Zhang, Jiakun,Zhao, Caiyan,Wu, Yan.

[2]小立碗藓细胞色素P450基因PpCYP51G1的分离及转化载体构建. 丁谦,叶帅,李景娟,朱正歌,高建伟. 2013

[3]Prochloraz采后处理对苹果贮藏期病害的控制. 盛占武,鄯晋晓,毕阳,葛永红,孙志高. 2006

[4]The Y123H substitution perturbs FvCYP51B function and confers prochloraz resistance in laboratory mutants of Fusarium verticillioides. Chen, F.,Diao, Y.,Liu, X.,Fan, J.,Chen, F.,Cools, H. J.,Kelly, S. L.,Kelly, S. L.. 2014

[5]Identification and haplotype analysis of the gene SiLfl1 regulate leaf length in foxtail millet. Yannan Wang,Han Jia,Linlin Zhang,Hui Zhi,Guanqing Jia,Xianmin Diao,Sha Tang. 2025

[6]Size-dependent effect of prochloraz-loaded mPEG-PLGA micro-and nanoparticles. Huang, Qiliang,Zhang, Jiakun,Cao, Lidong. 2014

[7]Transcriptomic dissection of the rice-Fusarium fujikuroi interaction by RNA-Seq. Ji, Zhijuan,Zeng, Yuxiang,Liang, Yan,Qian, Qian,Yang, Changdeng,Ji, Zhijuan,Qian, Qian.

[8]Genome-Wide Identification and Functional Analysis of the bZIP Transcription Factor Family in Rice Bakanae Disease Pathogen, Fusarium fujikuroi. Zhao, Kehan,Liu, Lianmeng,Huang, Shiwen. 2022

[9]Dissipation behavior of prochloraz and its metabolites in grape under open-field, storage and the wine-making process. Tian F.,Qiao C.,Wang C.,Pang T.,Guo L.,Li J.,Pang R.,Xie H.. 2022

[10]Functional analysis of all succinate dehydrogenase subunits in Fusarium fujikuroi. Mengyuan Fan,Hao Qi,Wenyong Shao,Hao Zhang,Yanni Yin,Yun Chen,Youfu Zhao,Zhonghua Ma. 2024

[11]Preparation and Synergistic Effect of Composite Solid Nanodispersions for Co-Delivery of Prochloraz and Azoxystrobin. Tingyu Wang,Fangyuan Chen,Xiquan Ding,Fei Gao,Qian Du,Zhanghua Zeng,Haixin Cui,Bo Cui. 2025

[12]Expression of a radish defensin in transgenic wheat confers increased resistance to Fusarium graminearum and Rhizoctonia cerealis. Li, Zhao,Zhang, Zengyan,Du, Lipu,Xu, Huijun,Xin, Zhiyong,Li, Zhao,Zhou, Miaoping,Ren, Lijuan,Zhang, Boqiao.

[13]Molecular cytogenetic analysis of a durum wheat x Thinopyrum distichum hybrid used as a new source of resistance to Fusarium head blight in the greenhouse. Chen, Q,Eudes, F,Conner, RL,Graf, R,Comeau, A,Collin, J,Ahmad, F,Zhou, R,Li, H,Zhao, Y,Laroche, A. 2001

[14]Molecular characterization of a novel mycovirus of the family Tymoviridae isolated from the plant pathogenic fungus Fusarium graminearum. Lin, Yanhong,Zhang, Hailong,Wang, Shuangchao,Qiu, Dewen,Guo, Lihua.

[15]Transcriptome-Based Discovery of Fusarium graminearum Stress Responses to FgHV1 Infection. Zhang, Jingze,Li, Pengfei,Qiu, Dewen,Guo, Lihua,Wang, Shuangchao. 2016

[16]A novel virus in the family Hypoviridae from the plant pathogenic fungus Fusarium graminearum. Liu, Liang,Guo, Lihua,Qiu, Dewen,Kondo, Hideki.

[17]Molecular characterization of a novel hypovirus from the plant pathogenic fungus Fusarium graminearum. Zhang, Hailong,Chen, Xiaoguang,Qiu, Dewen,Guo, Lihua.

[18]The Fusarium Graminearum Histone Acetyltransferases A.re Important For Morphogenesis, D on Biosynthesis, And Pathogenicity. Xu, Jingsheng,Zhang, Hao,Chen, Wanquan,Xu, Jin,Feng, Jie,van Diepeningen, Anne D.,van der Lee, Theo A. J.,Waalwijk, Cees,Kong, Xiangjiu. 2018

[19]Polyurea microcapsule encapsulation improves the contact toxicity, inhibition time and control effect of trans-2-hexenal against Fusarium graminearum. Dicheng Ma,Tao Zhang,Guoxian Wang,Chong Cao,Wei Mu,Beixing Li,Daolong Dou,Feng Liu. 2023

[20]Optimization For The Production Of D.eoxynivalenol And Zearalenone By F usarium Graminearum Using Response Surface Methodology. Wu, L, Qiu, LJ, Zhang, HJ, Sun, J, Hu, XX, Wang, BJ. 2017

作者其他论文 更多>>