数字农科院2.0

A functional study reveals CsNAC086 regulated the biosynthesis of flavonols in Camellia sinensis

文献类型： 外文期刊

作者： Sa Sa Song;Wei Xi Ran;Long Han Gao;Yu Chun Wang;Wu Yun Lv;Yu Tao;Liang Chen;Chun Fang Li

作者机构：

关键词： Antisense oligonucleotides;Camellia sinensis;Flavonol synthase;Flavonols;NAC transcription factor

期刊名称： Planta

ISSN： 0032-0935

年卷期： 2024 年 259 卷 6 期

页码：

收录情况： SCIE（2024版）

摘要： Main conclusion: CsNAC086 was found to promote the expression of CsFLS, thus promoting the accumulation of flavonols in Camellia sinensis. Abstract: Flavonols, the main flavonoids in tea plants, play an important role in the taste and quality of tea. In this study, a NAC TF gene CsNAC086 was isolated from tea plants and confirmed its regulatory role in the expression of flavonol synthase which is a key gene involved in the biosynthesis of flavonols in tea plant. Yeast transcription-activity assays showed that CsNAC086 has self-activation activity. The transcriptional activator domain of CsNAC086 is located in the non-conserved C-terminal region (positions 171–550), while the conserved NAC domain (positions 1–170) does not have self-activation activity. Silencing the CsNAC086 gene using antisense oligonucleotides significantly decreased the expression of CsFLS. As a result, the concentration of flavonols decreased significantly. In overexpressing CsNAC086 tobacco leaves, the expression of NtFLS was significantly increased. Compared with wild-type tobacco, the flavonols concentration increased. Yeast one-hybrid assays showed CsNAC086 did not directly regulate the gene expression of CsFLS. These findings indicate that CsNAC086 plays a role in regulating flavonols biosynthesis in tea plants, which has important implications for selecting and breeding of high-flavonols-concentration containing tea-plant cultivars.

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