数字农科院2.0

MatE transporter affects methane metabolism in Methermicoccus shengliensis and is modulated by methoxylated aromatic compounds

文献类型： 外文期刊

作者： Leng, Huan;Wang, Dong;Yang, Qing;Wang, Shuxin;Guo, Leizhou;Zhao, Pengyan;Chen, Yi;Dai, Lirong;Cha, Guihong;Bai, Liping;Delvigne, Frank

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期刊名称： COMMUNICATIONS BIOLOGY

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年卷期： 2025 年 8 卷 1 期

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收录情况： SCIE（2025版）

摘要： Methoxylated aromatic compounds, are abundant in subsurface ecosystems. Recently, it was discovered that Methermicoccus shengliensis can convert methoxylated aromatics to methane. Specifically, the MATE family transporters (MatE) and transduction-like protein (Tlp) were hypothesized to play a crucial role in substrate transport. However, their biological function and the transporting model remained unclear. To address this knowledge gap, we employed bacterial two-hybrid and structural model assays to investigate the interaction between Tlp and MatE. Our results revealed that Tlp senses 2-methoxybenzoate and interacts with MatE to facilitate substrate transport. Furthermore, we observed that the matE knock-out mutant significantly impaired the growth and methane production of M. shengliensis when using 2-methoxybenzoate as a substrate, highlighting the essential role of MatE in methoxydotrophic methanogenesis. Overall, our findings suggest that the MatE-Tlp system regulates substrate uptake and methane metabolism in M. shengliensis, providing new avenues for reducing global methane emissions caused by methanogens.

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