数字农科院2.0

Three-Dimensional Quantitative Coherent Diffraction Imaging of Staphylococcus aureus Treated with Peptide-Mineralized Au-Cluster Probes

文献类型： 外文期刊

作者： Li T.; He B.; Zhang X.; Fan J.; Gao L.; Sun Z.; Zhang J.; Guo A.; Pan D.; Yin X.; Tong Y.; Song C.; Kohmura Y.; Yabashi M.; Ishikawa T.; Gao X.; Jiang H.

作者机构：

关键词： Bacteria; Cell membranes; Cell proliferation; Drug interactions; Probes; Structural analysis; Three dimensional computer graphics; Au clusters; Cell-be; Cell/B.E; Characterization methods; Coherent diffraction imaging; Functional metals; Intact cells; Metal nanoclusters; Microbial cells; Staphylococcus aureus; Peptides

期刊名称： Analytical Chemistry

ISSN： 0003-2700

年卷期： 2022 年

页码：

收录情况： SCIE（2022版） ; EI（2022版）

摘要： Characterizing interactions between microbial cells and their specific inhibitory drugs is essential for developing effective drugs and understanding the therapeutic mechanism. Functional metal nanoclusters can be effective inhibitory agents against microorganisms according to various characterization methods, but quantitative three-dimensional (3D) spatial structural analysis of intact cells is lacking. Herein, using coherent X-ray diffraction imaging, we performed in situ 3D visualization of unstained Staphylococcus aureus cells treated with peptide-mineralized Au-cluster probes at a resolution of ~47 nm. Subsequent 3D mass-density mapping and quantitative structural analyses of S. aureus in different degrees of destruction showed that the bacterial cell wall was damaged and cytoplasmic constituents were released from cells, confirming the significant antibacterial effects of the Au-cluster probe. This study provides a promising nondestructive approach for quantitative imaging and paves the way for further research into microbe-inhibitor drug interactions. © 2022 American Chemical Society.

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